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dc.contributorVechalapu, Suryanarayanaen_US
dc.contributorVarshney, Rajeeven_US
dc.contributorSingh, Vikasen_US
dc.contributorSaxena, Rachiten_US
dc.contributorSameer Kumar, Chanda Venkataen_US
dc.contributorKatta, Mohan AVSen_US
dc.contributorKhan, Awaisen_US
dc.contributorSaxena, KBen_US
dc.creatorPallavi, Sinhaen_US
dc.identifier.citationSinha Pallavi, Suryanarayana Vechalapu, Rajeev Varshney, Vikas Singh, Rachit Saxena, Chanda Venkata Sameer Kumar, Mohan AVS Katta, Awais Khan, KB Saxena. (15/7/2015). Association of nad7a Gene with Cytoplasmic Male Sterility in Pigeonpea. Plant Genome, 8(2), pp. 1-12.en_US
dc.description.abstractCytoplasmic male sterility (CMS) has been exploited in the commercial pigeonpea [Cajanus cajan (L.) Millsp.] hybrid breeding system; however, the molecular mechanism behind this system is unknown. To understand the underlying molecular mechanism involved in A4 CMS system derived from C. cajanifolius (Haines) Maesen, 34 mitochondrial genes were analyzed for expression profiling and structural variation analysis between CMS line (ICRISAT Pigeonpea A line, ICPA 2039) and its cognate maintainer (ICPB 2039). Expression profiling of 34 mitochondrial genes revealed nine genes with significant fold differential gene expression at P ≤ 0.01, including one gene, nad4L, with 1366-fold higher expression in CMS line as compared with the maintainer. Structural variation analysis of these mitochondrial genes identified length variation between ICPA 2039 and ICPB 2039 for nad7a (subunit of nad7 gene). Sanger sequencing of nad4L and nad7a genes in the CMS and the maintainer lines identified two single nucleotide polymorphisms (SNPs) in upstream region of nad4L and a deletion of 10 bp in nad7a in the CMS line. Protein structure evaluation showed conformational changes in predicted protein structures for nad7a between ICPA 2039 and ICPB 2039 lines. All above analyses indicate association of nad7a gene with the CMS for A4 cytoplasm in pigeonpea. Additionally, one polymerase chain reaction (PCR) based Indel marker (nad7a_del) has been developed and validated for testing genetic purity of A4 derived CMS lines to strengthen the commercial hybrid breeding program in pigeonpea.en_US
dc.publisherCrop Science Society of Americaen_US
dc.sourcePlant Genome;8,(2015) Pagination 1,12en_US
dc.subjecthybrid breedingen_US
dc.titleAssociation of nad7a Gene with Cytoplasmic Male Sterility in Pigeonpeaen_US
dc.typeJournal Articleen_US
cg.subject.agrovocplant breedingen_US
cg.subject.agrovoccytoplasmic male sterilityen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerInternational Potato Center - CIPen_US
cg.contributor.crpCRP on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.contributor.project-lead-instituteInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
dc.identifier.statusOpen accessen_US

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