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dc.contributorMcPhee, Kevinen_US
dc.contributorAgrawal, Shiv Kumaren_US
dc.creatorSen Gupta, Debjyotien_US
dc.identifier.citationDebjyoti Sen Gupta, Kevin McPhee, Shiv Kumar Agrawal. (13/4/2017). Development of Molecular Markers for Iron Metabolism Related Genes in Lentil and Their Expression Analysis under Excess Iron Stress. Frontiers in Plant Science, 8: 579.en_US
dc.description.abstractMultiple genes and transcription factors are involved in the uptake and translocation of iron in plants from soil. The sequence information about iron uptake and translocation related genes is largely unknown in lentil (Lens culinaris Medik.). This study was designed to develop iron metabolism related molecular markers for Ferritin-1, BHLH-1 (Basic helix loop helix), or FER-like transcription factor protein and IRT-1 (Iron related transporter) genes using genome synteny with barrel medic (Medicago truncatula). The second objective of this study was to analyze differential gene expression under excess iron over time (2 h, 8 h, 24 h). Specific molecular markers were developed for iron metabolism related genes (Ferritin-1, BHLH-1, IRT-1) and validated in lentil. Gene specific markers for Ferritin-1 and IRT-1 were used for quantitative PCR (qPCR) studies based on their amplification efficiency. Significant differential expression of Ferritin-1 and IRT-1 was observed under excess iron conditions through qPCR based gene expression analysis. Regulation of iron uptake and translocation in lentil needs further characterization. Greater emphasis should be given to development of conditions simulating field conditions under external iron supply and considering adult plant physiology.en_US
dc.publisherFrontiers Mediaen_US
dc.sourceFrontiers in Plant Science;8:579,(2017)en_US
dc.subjectmolecular markeren_US
dc.subjectiron metabolismen_US
dc.subjectqpcr expression analysisen_US
dc.titleDevelopment of Molecular Markers for Iron Metabolism Related Genes in Lentil and Their Expression Analysis under Excess Iron Stressen_US
dc.typeJournal Articleen_US
cg.creator.idAgrawal, Shiv Kumar: 0000-0001-8407-3562en_US
cg.subject.agrovocgene expressionen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerIndian Council of Agricultural Research, Indian Institute of Pulses Research - ICAR-IIPRen_US
cg.contributor.centerNorth Dakota State University - NDSUen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderArab Fund for Economic and Social Development - AFESDen_US
cg.contributor.projectSustainability and Operation of the Regional Research Centers in a Number of Arab Countries (Phase II)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
cg.journalFrontiers in Plant Scienceen_US

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