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dc.contributorRijeibi, Mohammeden_US
dc.contributorAwadi, Sofiaen_US
dc.contributorRekik, Mouraden_US
dc.contributorGharbi, Mohameden_US
dc.creatorAmdouni, Yosraen_US
dc.identifier.citationYosra Amdouni, Mohammed Rijeibi, Sofia Awadi, Mourad Rekik, Mohamed Gharbi. (15/3/2018). First molecular detection and phylogenetic analyses of Neospora caninum from naturally infected sheep in North Africa.en_US
dc.description.abstractNeospora caninum is a protozoan parasite from the phylum Apicomplexa. This parasite is as one of the major causative abortive agents in the dairy cattle industry. Moreover, abortions in sheep due to N. caninum have been reported by several studies (Moreno et al, 2012). The objective of this study was to estimate the molecular prevalence and phylogenetic analyses of N. caninum in Tunisian sheep. A total number of 198 meat samples were collected from slaughtered ewes and tested for the presence of N. caninum ITS1 gene using PCR followed by sequencing of some PCR products. A phylogeneyic tree was then constructed to compare the partial sequences of the ITS1 gene with GenBank sequences. The overall N. caninum infection prevalence rate in sheep was 10.6±4.3% (21/198). The infection rate was significantly higher for animals aged over one year (19.4±9.1%) when compared to those under one year of age (5.6±4) (p<0.001). The highest prevalence was observed in North Béja locality (31.2±16.1) (p<0.001). Compared to Barbarine (6.8±4.5%) and Cross-bred animals (0%), Noire de Thibar was the most infectedsheep breed (31.7±14.2%) (p<0.001). Comparison of the partial sequences of the ITS1 gene revealed 96-98% similarity among our N. caninum amplicon and these deposited in GenBank. To our knowledge this is the first molecular study and phylogenetic analysis of N. caninum in sheep in North Africa. Our results indicate the meat harbour N. caninum cysts and can contribute to a better evaluation of N. caninum infection and the associated abortions in sheep.en_US
dc.titleFirst molecular detection and phylogenetic analyses of Neospora caninum from naturally infected sheep in North Africaen_US
dc.typeConference Paperen_US
cg.creator.idRekik, Mourad: 0000-0001-7455-2017en_US
cg.subject.agrovocphylogenetic analysisen_US
cg.subject.agrovocneospora caninumen_US
cg.contributor.centerManouba University, National School of Veterinary Medicine of Sidi Thabet - ENMVen_US
cg.contributor.centerRegional Slaughterhouse of Béjaen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.crpCGIAR Research Program on Livestock Agri-Food Systems - LAFSen_US
cg.contributor.funderInternational Livestock Research Institute - ILRIen_US
cg.contributor.projectCGIAR Research Program on Livestock Agri-Food Systemsen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionNorthern Africaen_US
dc.identifier.statusOpen accessen_US

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