Evaluation of various methods for the detection of barley yellow dwarf virus by the tissue-blot immunoassay and its use for virus detection in cereals inoculated at different growth stages
cg.contact | k.makkouk@cgiar.org | en_US |
cg.contributor.center | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.contributor.funder | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.contributor.project | Communication and Documentation Information Services (CODIS) | en_US |
cg.contributor.project-lead-institute | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.date.embargo-end-date | Timeless | en_US |
cg.identifier.doi | https://dx.doi.org/10.1007/BF01871967 | en_US |
cg.isijournal | ISI Journal | en_US |
cg.issn | 0929-1873 | en_US |
cg.issn | 1573-8469 | en_US |
cg.journal | European Journal of Plant Pathology | en_US |
cg.subject.agrovoc | barley | en_US |
cg.subject.agrovoc | wheat | en_US |
cg.subject.agrovoc | hordeum vulgare | en_US |
cg.subject.agrovoc | triticum aestivum | en_US |
cg.subject.agrovoc | serology | en_US |
cg.subject.agrovoc | avena sativa | en_US |
cg.volume | 100 | en_US |
dc.contributor | Comeau, A. | en_US |
dc.creator | Makkouk, Khaled | en_US |
dc.date.accessioned | 2021-06-15T20:33:27Z | |
dc.date.available | 2021-06-15T20:33:27Z | |
dc.description.abstract | Various modifications of the tissue-blot immunoassay (TBIA) for the detection of barley yellow dwarf virus (BYDV, luteovirus) were compared. Similar results were obtained by using three different labelled molecules; goat anti-rabbit antibodies conjugated to alkaline phosphatase, protein A conjugated with alkaline phosphatase and goat anti-rabbit antibodies conjugated with colloidal gold. Blocking the nitrocellulose membrane with polyvinyl alcohol for 1 min was effective and allowed the procedure to be shortened by one hour. TBIA was sensitive enough to detect BYDV in old dry tissue wich had been soaked in water for 1 h. BYDV was monitored by TBIA in wheat, oat and barley after inoculation at heading, flowering and grain filling growth stages. The later the inoculation date, the greater the chance of detecting the virus in stem bases rather than in the upper part of the stem. The later the inoculation the less virus moved, from the inoculated tiller to other tillers of the same plant. | en_US |
dc.identifier | https://mel.cgiar.org/dspace/limited | en_US |
dc.identifier.citation | Khaled Makkouk, A. Comeau. (1/4/1994). Evaluation of various methods for the detection of barley yellow dwarf virus by the tissue-blot immunoassay and its use for virus detection in cereals inoculated at different growth stages. European Journal of Plant Pathology, 100, pp. 71-80. | en_US |
dc.identifier.status | Timeless limited access | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/13217 | |
dc.language | en | en_US |
dc.publisher | Springer (part of Springer Nature) | en_US |
dc.source | European Journal of Plant Pathology;100,(1994) Pagination 71-80 | en_US |
dc.subject | oat | en_US |
dc.title | Evaluation of various methods for the detection of barley yellow dwarf virus by the tissue-blot immunoassay and its use for virus detection in cereals inoculated at different growth stages | en_US |
dc.type | Journal Article | en_US |
dcterms.available | 1994-04-01 | en_US |
dcterms.extent | 71-80 | en_US |
mel.impact-factor | 1.582 | en_US |