Evaluation of various methods for the detection of barley yellow dwarf virus by the tissue-blot immunoassay and its use for virus detection in cereals inoculated at different growth stages

cg.contactk.makkouk@cgiar.orgen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.date.embargo-end-dateTimelessen_US
cg.identifier.doihttps://dx.doi.org/10.1007/BF01871967en_US
cg.isijournalISI Journalen_US
cg.issn0929-1873en_US
cg.issn1573-8469en_US
cg.journalEuropean Journal of Plant Pathologyen_US
cg.subject.agrovocbarleyen_US
cg.subject.agrovocwheaten_US
cg.subject.agrovochordeum vulgareen_US
cg.subject.agrovoctriticum aestivumen_US
cg.subject.agrovocserologyen_US
cg.subject.agrovocavena sativaen_US
cg.volume100en_US
dc.contributorComeau, A.en_US
dc.creatorMakkouk, Khaleden_US
dc.date.accessioned2021-06-15T20:33:27Z
dc.date.available2021-06-15T20:33:27Z
dc.description.abstractVarious modifications of the tissue-blot immunoassay (TBIA) for the detection of barley yellow dwarf virus (BYDV, luteovirus) were compared. Similar results were obtained by using three different labelled molecules; goat anti-rabbit antibodies conjugated to alkaline phosphatase, protein A conjugated with alkaline phosphatase and goat anti-rabbit antibodies conjugated with colloidal gold. Blocking the nitrocellulose membrane with polyvinyl alcohol for 1 min was effective and allowed the procedure to be shortened by one hour. TBIA was sensitive enough to detect BYDV in old dry tissue wich had been soaked in water for 1 h. BYDV was monitored by TBIA in wheat, oat and barley after inoculation at heading, flowering and grain filling growth stages. The later the inoculation date, the greater the chance of detecting the virus in stem bases rather than in the upper part of the stem. The later the inoculation the less virus moved, from the inoculated tiller to other tillers of the same plant.en_US
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifier.citationKhaled Makkouk, A. Comeau. (1/4/1994). Evaluation of various methods for the detection of barley yellow dwarf virus by the tissue-blot immunoassay and its use for virus detection in cereals inoculated at different growth stages. European Journal of Plant Pathology, 100, pp. 71-80.en_US
dc.identifier.statusTimeless limited accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/13217
dc.languageenen_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.sourceEuropean Journal of Plant Pathology;100,(1994) Pagination 71-80en_US
dc.subjectoaten_US
dc.titleEvaluation of various methods for the detection of barley yellow dwarf virus by the tissue-blot immunoassay and its use for virus detection in cereals inoculated at different growth stagesen_US
dc.typeJournal Articleen_US
dcterms.available1994-04-01en_US
dcterms.extent71-80en_US
mel.impact-factor1.582en_US

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