Evaluation of Broad-Spectrum Streptomyces sp. for Plant Growth Promotion Traits in Chickpea (Cicer arietinum L.)
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Citation
Subramaniam Gopalakrishnan, Prakash Gangashetty, Hima bindu Kudapa, Rajeev Varshney, Srinivas Vadlamudi, Gottumukkala Alekhya. (28/9/2015). Evaluation of Broad-Spectrum Streptomyces sp. for Plant Growth Promotion Traits in Chickpea (Cicer arietinum L. ). The Philippine Agricultural Scientist, 98 (3), pp. 270-278.
Abstract
The study evaluated three strains of Streptomyces (CAI-21, CAI-26 and MMA-32) for their plant
growth promoting (PGP) traits in chickpea (Cicer arietinum L.) under field conditions in two
consecutive post-rainy seasons, 2012–2013 and 2013–2014, to confirm colonizing ability in
chickpea and to demonstrate gene expression profiles of indoleacetic acid, siderophore and β-
1,3-glucanase genes. The Streptomyces enhanced nodule number, nodule weight, root weight
and shoot weight at 30 d after sowing (DAS) and plant height, pod number, pod weight, leaf
area, leaf weight and stem weight at 60 DAS in both seasons over the uninoculated control. At
crop maturity, the Streptomyces enhanced stover yield, grain yield and total dry matter in both
seasons over the uninoculated control. In the rhizosphere, the biological and mineral nutrient
activities, such as microbial biomass carbon, dehydrogenase activity, total nitrogen, available
phosphorus and organic carbon were also found higher in plots inoculated with Streptomyces
in both seasons over the uninoculated control plots. Scanning electron microscopy analysis
revealed that the Streptomyces colonized the roots of chickpea. Quantitative real time PCR
analysis on selected PGP genes of Streptomyces revealed up-regulation of β-1,3-glucanase
gene in CAI-26, β-1,3-glucanase and siderophore genes (siderophore synthetase) in CAI-21 and
β-1,3-glucanase, siderophore (siderophore synthetase) and indole-3-acetic acid (IAAH and
IAAM) genes in MMA-32. The three Streptomyces demonstrated broad-spectrum PGP activity.
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Author(s) ORCID(s)
Gopalakrishnan, Subramaniam https://orcid.org/0000-0003-4053-7016
Gangashetty, Prakash https://orcid.org/0000-0002-6766-1415
Gangashetty, Prakash https://orcid.org/0000-0002-6766-1415