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dc.contributorYahyaoui, Amoren_US
dc.contributorRezgui, Salahen_US
dc.contributorElias, Elias M.en_US
dc.contributorDaaloul, Abderrazaken_US
dc.creatorFakhfakh, Men_US
dc.identifier.citationM Fakhfakh, Amor Yahyaoui, Salah Rezgui, Elias M. Elias, Abderrazak Daaloul. (11/7/2011). Identification and pathogenicity assessment of Fusarium spp. sampled from durum wheat fields in Tunisia. African journal of biotechnology, 10 (34), pp. 6529-6539.en_US
dc.description.abstractDurum wheat is the major cereal crop cultivated in Tunisia; covering over 40% of the cereal growing areas. Durum wheat production remains below expectation due to its low productivity that is attributed to the chronically abiotic and biotic stresses. Fusarium head blight (FHB) caused by Fusarium spp. has become an important disease on durum wheat (Triticum turgidum L var. durum) in Tunisia, particularly during humid season. To identity the Fusarium species associated with FHB, samples were collected from five and six durum wheat fields in Northern Tunisia during 2004 and 2007 crop seasons, respectively. A total of 241 single spore cultures were isolated and seven different Fusarium spp. were identified using morphological traits and species-specific PCR assays. Pathogenicity of FHB causal agents was assessed on predominant durum wheat varieties. Aggressiveness of selected Fusarium isolates was investigated. Three durum wheat cultivars, characterized by different level of susceptibility to FHB, were artificially inoculated. Symptoms of FHB were rated as percentage of infected spikelets (PIS) at 7, 14 and 21 days after inoculation (DAI) and area under disease progress curve (AUDPC) was analysed. Fusarium culmorum was the dominant species representing 36.3% of all sampled isolates. All Fusarium species tested caused visible infections to the durum wheat cultivars with significant difference in aggressiveness among species. F. culmorum was considered highly pathogenic with an AUDPC = 243.7, followed by Fusarium pseudograminearum (AUDPC = 216.7). The remaining species, which had < 150 AUDPC, were moderately and weakly pathogenic. There were significant differences (P < 0.05) in aggressiveness among isolates within species suggesting that screening for resistance to FHB requires a mixture of several isolates within and among Fusarium species.en_US
dc.publisherAcademic Journalsen_US
dc.sourceAfrican journal of biotechnology;10,(2011) Pagination 6529-6539en_US
dc.subjectfusarium head blight (fhb)en_US
dc.subjectfusarium speciesen_US
dc.titleIdentification and pathogenicity assessment of Fusarium spp. sampled from durum wheat fields in Tunisiaen_US
dc.typeJournal Articleen_US
cg.subject.agrovocdurum (triticum durum)en_US
cg.subject.agrovocDurum Wheaten_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerNational Agronomic Institute of Tunisia - INATen_US
cg.contributor.centerNorth Dakota State University - NDSUen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionNorthern Africaen_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
cg.journalAfrican journal of biotechnologyen_US

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