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Multi environment evaluation of chickpea disease nurseries (wilt, Aschochyta blight and Botrytis grey mold) to identify stable and new sources of resistance
Under the ICRISAT- ICAR collaboration, disease nurseries of wilt (ICWN), AB (IABN) and BGM (IBGMN) consisting of 30 entries (28 resistant cultivars and 2 susceptible checks) were evaluated at different locations (hot spots) ...
Develop MAGIC and biparental populations following SSD (2) Phenotyping of populations for target traits
Seed increase is in progress of 3000 F6 generation MAGIC lines derived from the crosses involving the parents, ICGV 91114, ICGV 06040, 55-437, ICGV 00440, ICGV 00308, GPBD 4, ICGV 05155 and ICGV 88145. RIL population derived ...
Quantification of Foc colonization through qPCR in resistant and susceptible cultivars grown under elevated CO2
The chickpea seedlings of susceptible cv. JG 62 and resistant cv. WR 315 were grown in open top chambers at different CO2 concentrations [ambient (~380 ppm) and elevated ( 550 ppm and 700 ppm). Seven day old seedlings were ...
Multi-environment evaluation of pigeonpea disease nursery of 28 lines to wilt and SMD resistance
Under the ICAR-ICRISAT collaboration, during 2015-16 crop seasons, Pigeonpea Wilt and SM Disease Nursery (PWSMDN) consisting of 30 entries (28 resistant cultivars and 2 susceptible checks) were evaluated for wilt and SMD ...
Development of proteome library from FOC inoculated chickpea resistant and susceptible cultivars under elevated CO2 through MALDI and MS-MS analysis
Total root proteins of control and Foc infected chickpea (JG 62 and WR 315) were extracted. Isoelectric focusing (IEF) of extracted proteins was carried out using immobilized pH gradient (IPG) strips. 2-DE gels were scanned ...
Expression profiling from different tissues and developmental stages to define gene atlas
Thirty seven different samples representing 19 aerial, seven underground, two germinal and nine seed development related tissues from five different developmental stages have been targeted. The five developmental stages ...
Surveys conducted to know the occurrence and distribution of diseases in chickpea and pigeonpea growing regions
For chickpea disease, a survey was conducted between November and January in four major chickpea growing states in central and southern parts of India viz. Andhra Pradesh, Karnataka and Maharashtra. For pigeonpea, survey ...
RNA-seq based transcriptome profiling, identification and validation of heat stress responsive genes in chickpea
Six contrasting heat responsive (tolerant - ICCV 92944, ICC 1356, ICC 15614 and sensitive - ICC 5912, ICC 4567, ICC 10685) genotypes were selected for the study. Heat stress was imposed using growth chamber facility, ...
Study of expression pattern of defence genes in chickpea and pathogenicity causing genes in FOC through qPCR in resistant and susceptible cultivars grown under elevated CO2
Total RNA was extracted from root tissues of wilt infected and control chickpea seedlings of susceptible cv. JG 62 and resistant cv. WR 315 grown under ambient and elevated CO2 condition. cDNA was synthesized from total ...
Temperature sensitivity of food legumes: a physiological insight
Of the various environmental stresses that a
plant can experience, temperature has the widest and most
far-reaching effects on legumes. Temperature extremes,
both high (heat stress) and low (cold stress), are injurious ...