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dc.contributorAre, Ashok Kumaren_US
dc.contributorAgrawal, Shiv Kumaren_US
dc.contributorKemal, Seid Ahmeden_US
dc.contributorGill, Ranjit Kauren_US
dc.contributorSingh, Sarvjeeten_US
dc.contributorSharma, Tilak Rajen_US
dc.creatorFikru, Mekonnenen_US
dc.identifier en_US
dc.identifier.citationMekonnen Fikru, Ashok Kumar, Shiv Kumar Agrawal, Seid Kemal, Ranjit Kaur Gill, Sarvjeet Singh, Tilak Raj Sharma. (31/12/2016). Identification of molecular markers associated with rust resistance genes in lentil (Lens culinaris sub sp. culinaris).en_US
dc.description.abstractLentil rust caused by Uromyces vicia- fabae (Pers.) J. Schroet is the most widespread and economically important disease in Ethiopia, Bangladesh, India, Nepal, and Morocco. The objective of this study was to determine the genetics of rust resistance in lentil and to locate map position of gene(s) associated with resistance to rust and to identify molecular markers that can be used in marker-assisted selection. We report here the mapping of rust resistance gene using lentil specific SSR markers. For this purpose, a recombinant inbred line (RILs) population comprising of 133 lines, derived from the cross of a rust resistant line (FLIP-2004-7L) as male parent, and a rust susceptible line (L-9-12) as female parent was used. Phenotyping of the RIL population was carried out at rust hot spots in Chefe Donsa, Sinana and Sirinka (Ethiopia) during summers of 2011 and 2012 and at Gurdaspur, India during 2011/12 cropping season. The mapping population segregated in 1:1 ratio (χ2= 0.008), indicating that lentil rust resistance is controlled by a single gene. Linkage analysis for rust resistance gene showed that the SSR marker GLLC106 was located at a distance of 10cM from resistance gene. All resistant RILs had bands at 200bp region. This co-segregating marker can be used for marker-assisted selection in a lentil breeding program aimed at developing lentil cultivars resistant to rust disease. However, future research should be aimed at developing additional markers closer to the resistance gene so that the region is saturated.en_US
dc.subjectpests and diseaseen_US
dc.titleIdentification of molecular markers associated with rust resistance genes in lentil (Lens culinaris sub sp. culinaris)en_US
cg.creator.idAgrawal, Shiv Kumar: 0000-0001-8407-3562en_US
cg.creator.idKemal, Seid Ahmed: 0000-0002-1791-9369en_US
cg.contributor.centerWollo University - WUen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerPunjab Agricultural University - PAUen_US
cg.contributor.crpCRP on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionWestern Africaen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.regionEastern Africaen_US
dc.identifier.statusOpen accessen_US

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