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dc.contributorRoorkiwal, Manishen_US
dc.contributorKale, Sandipen_US
dc.contributorVanika, Gargen_US
dc.contributorYadala, Ramakrishnaen_US
dc.contributorVarshney, Rajeeven_US
dc.creatorJain, Ankiten_US
dc.identifier.citationAnkit Jain, Manish Roorkiwal, Sandip Kale, Garg Vanika, Ramakrishna Yadala, Rajeev Varshney. (18/3/2019). InDel markers: An extended marker resource for molecular breeding in chickpea. PLoS ONE, 3(14), pp. 1-13.en_US
dc.description.abstractChickpea is one of the most important food legumes that holds the key to meet rising global food and nutritional demand. In order to deploy molecular breeding approaches in crop improvement programs, user friendly and cost effective marker resources remain prerequisite. The advent of next generation sequencing (NGS) technology has resulted in the generation of several thousands of markers as part of several large scale genome sequencing and re-sequencing initiatives. Very recently, PCR based Insertion-deletions (InDels) are becoming a popular gel based genotyping solution because of their co-dominant, inexpensive, and highly polymorphic nature. With an objective to expand marker resources for genomics assisted breeding (GAB) in chickpea, whole genome re-sequencing data generated on five parental lines of one interspecific (ICC 4958 × PI 489777) and two intra-specific (ICC 283 × ICC 8261 and ICC 4958 × ICC 1882) mapping populations, were used for identification of InDels. A total of 231,658 InDels were identified using Dindel software with default parameters. Further, a total of 8,307 InDels with ≥20 bp size were selected for development of gel based markers, of which primers could be designed for 7,523 (90.56%) markers. On average, markers appeared at a frequency of 1,038 InDels/LG with a maximum number of markers on CaLG04 (1,952 InDels) and minimum on CaLG08 (360 InDels). In order to validate these InDels, a total of 423 primer pairs were randomly selected and tested on the selected parental lines. A high amplification rate of 80% was observed ranging from 46.06 to 58.01% polymorphism rate across parents on 3% agarose gel. This study clearly reflects the usefulness of available sequence data for the development of genome-wide InDels in chickpea that can further contribute and accelerate a wide range of genetic and molecular breeding activities in chickpea.en_US
dc.publisherPublic Library of Scienceen_US
dc.sourcePLoS ONE;3,(2019) Pagination 1,13en_US
dc.subjectgenetic studiesen_US
dc.subjectchickpea improvementen_US
dc.subjectsequencing dataen_US
dc.subjectgenome-wide markersen_US
dc.subjectindel markersen_US
dc.subjectchickpea accessionsen_US
dc.titleInDel markers: An extended marker resource for molecular breeding in chickpeaen_US
dc.typeJournal Articleen_US
cg.creator.idRoorkiwal, Manish: 0000-0001-6595-281Xen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.crpCRP on Grain Legumes and Dryland Cereals - GLDCen_US
cg.contributor.funderMinistry of Science and Technology, Department of Biotechnology - MOST - DBTen_US
cg.contributor.projectImproving Chickpea Adaptation to Environmental Challenges in Australia and Indiaen_US
cg.contributor.project-lead-instituteInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.coverage.regionWestern Europeen_US
cg.coverage.regionSouthern Asiaen_US
cg.isijournalISI journalen_US
dc.identifier.statusOpen accessen_US
cg.journalPLoS ONEen_US

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