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dc.contributorGupta, Priyankaen_US
dc.contributorMahmood Khawar, Khaliden_US
dc.contributorOzcan, Sebahattinen_US
dc.contributorAgrawal, Shiv Kumaren_US
dc.creatorBarpete, Surendraen_US
dc.identifier.citationSurendra Barpete, Priyanka Gupta, Khalid Mahmood Khawar, Sebahattin Ozcan, Shiv Kumar Agrawal. (1/4/2020). In vitro Approaches for Shortening Generation Cycles and Faster Breeding of low β-n-oxalyl-l-α, β-diamino propionic acid content Grass pea (Lathyrus sativus L. ). Fresenius Environmental Bulletin, 29 (4), pp. 2698-2706.en_US
dc.description.abstractGrass pea (Lathyrus sativus L.) grows as long day plant under natural conditions with single breeding cycle per annum. There is a need to develop strategies by accelerating breeding activities, making it possible to grow more than one generation per annum. This study aimed to search alternative in vitro mopho-physiological approaches to induce earlier flowering and accelerate breeding cycles. Nodal junctions with internodes were used as explants to multiple shoots using different concentrations of 6-benzylaminopurine (BAP) and αnaphthalene acetic acid (NAA). The MS medium supplemented with 2 mg L-1 BAP and 1 mg L-1 NAA was most optimum for production of multiple shoots. These shoots were transferred to MS medium containing 0.1- 0.50 mg L-1 BAP singly for generative transformation and growth. The MS medium supplemented with 0.25 mg L-1 BAP was most optimum for induced floral buds and flowers within two weeks of culture showing an average number of 4.30± 0.32 flowers per plantlet that continued and increased in number during rooting and acclimatization. The plantlets did not face any problem during acclimatization in the greenhouse, where they set seeds. The maximum β-N-oxalyl-l-α, βdiaminopropionic acid (β-ODAP) accumulation was recorded (1.48%) at early developmental stage followed by the flowering stage of grass pea. The results are very encouraging and the methodology used in this experiment could positively contribute towards accelerated breeding of ODAP free grass pea varieties.en_US
dc.sourceFresenius Environmental Bulletin;29,Pagination 2698,2706en_US
dc.subjectin vitro floweringen_US
dc.subjectnodal junctionen_US
dc.subjectlathyrus sativus l.en_US
dc.subjectGrass pea (Lathyrus sativus)en_US
dc.titleIn vitro Approaches for Shortening Generation Cycles and Faster Breeding of low β-n-oxalyl-l-α, β-diamino propionic acid content Grass pea (Lathyrus sativus L.).en_US
dc.typeJournal Articleen_US
cg.creator.idBarpete, Surendra: 0000-0002-5547-8488en_US
cg.creator.idAgrawal, Shiv Kumar: 0000-0001-8407-3562en_US
cg.subject.agrovocgrass peaen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerAnkara University, Faculty of Agriculture - AU - FoAen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes and Dryland Cereals - GLDCen_US
cg.contributor.funderIndian Council of Agricultural Research - ICARen_US
cg.contributor.projectIndia Collaborative Program: Restricted funding for breeding for resistance to abiotic stresses in pulses & for 2017/2018 - 2017/2020 - 2020/2021en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
dc.identifier.statusOpen accessen_US

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