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dc.contributorWaeyenberge, Lievenen_US
dc.contributorViaene, Nicoleen_US
dc.contributorDababat, Ameren_US
dc.contributorNicol, Julieen_US
dc.contributorOgbonnaya, Francis Chuksen_US
dc.contributorMoens, Mauriceen_US
dc.creatorToumi, Fatehen_US
dc.identifier.citationFateh Toumi, Lieven Waeyenberge, Nicole Viaene, Amer Dababat, Julie Nicol, Francis Chuks Ogbonnaya, Maurice Moens. (10/3/2013). Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi. European Journal of Plant Pathology, 136 (3), pp. 613-624.en_US
dc.description.abstractTwelve Heterodera species are of major economic significance in wheat and barley. Of these, H. avenae, H. filipjevi and H. latipons are among the most important ones, and sometimes coexist. The identification of Heterodera species using morphological characteristics is time consuming, requires specialized skill and can be imprecise, especially when they occur mixed in field populations. Molecular techniques can provide a more accurate way for nematode identification. This study reports the results of experiments targeting the mitochondrial cytochrome oxidase subunit 1 (COI) gene to develop species-specific primers that could be used for the identification of H. avenae and H. filipjevi. The COI gene of 9 Heterodera spp. and Punctodera punctata was partially sequenced and the resultant sequences were aligned to find unique sites suitable for the design of primers. The alignment showed variability between H. avenae, H. filipjevi and other Heterodera species. Two sets of species-specific primers were identified for the identification of both species and the conditions for their use in PCR were optimised. The specificity of the designed primers was checked by comparison with one population of P. punctata and populations of 14 other Heterodera species, nine populations of H. avenae and 10 populations of H. filipjevi originating from different countries. To test the sensitivity, the PCR was run with DNA extracted from five second-stage juveniles (J2) of H. avenae or five J2 of H. filipjevi mixed with DNA extracted from varying numbers of J2 of H. latipons. It was possible to detect as few as five J2 of H. avenae or H. filipjevi among 100 J2 of H. latipons. The two primers sets allow the detection of H. avenae and H. filipjevi where they occur in mixed populations with other Heterodera spp.en_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.sourceEuropean Journal of Plant Pathology;136,(2013) Pagination 613-624en_US
dc.subjectcereal cyst nematodesen_US
dc.titleDevelopment of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevien_US
dc.typeJournal Articleen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerInternational Maize and Wheat Improvement Center - CIMMYTen_US
cg.contributor.centerGhent University, Faculty of Bioscience Engineering, Department of Molecular Biotechnology - GU - BW - Biotechnologyen_US
cg.contributor.centerInstitute for Agricultural and Fisheries Research - ILVOen_US
cg.contributor.centerGhent University, Faculty of Bioscience Engineering - GU - BWen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionEastern Asiaen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.regionWestern Asiaen_US
cg.coverage.regionNorthern Africaen_US
cg.coverage.regionWestern Europeen_US
cg.isijournalISI Journalen_US
dc.identifier.statusTimeless limited accessen_US
cg.journalEuropean Journal of Plant Pathologyen_US

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