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dc.contributorKumari, Safaaen_US
dc.contributorKasem, Aminen_US
dc.contributorAl-Chaabi, Salahen_US
dc.contributorArab, Atehen_US
dc.creatorNader, Asaaden_US
dc.identifier.citationAsaad, N. S. G. Kumari, A. A. Haj-Kassem, S. Al-Chaabi and A. Arab. 2020. Developing an artificial inoculation technique for screening chickpea genotypes against a Syrian isolate of Chickpea chlorotic stunt virus. Arab Journal of Plant Protection, 38(2): 103-114.en_US
dc.description.abstractChickpea chlorotic stunt virus (CpCSV) is the most important virus affecting chickpea crop in several countries of West Asia and North Africa (WANA), including Syria. The main objective of this study was to develop a simple and economical technique to screen chickpea genotypes for virus resistance under open filed conditions. A total of 75 chickpea genotypes originated from 16 countries (obtained from the Gene Bank of ICARDA), including 3 local and 2 susceptible varieties, were used in this study. Thirty seeds of each genotype were sown in seedling tray plates containing a mixture of peat moss, sand and soil (1:0.5: 0.5). Two weeks later, seedlings were inoculated with a Syrian isolate of CpCSV using the aphid virus vector Aphis craccivora Koch under green house conditions. Twenty-five days after sowing, seedlings were transplanted into the field. Results showed that the method used showed high infection efficiency and can be used as a practical technique to screen chickpea genotypes for CpCSV resistance, as the virus infection rate reached 100% in many susceptible chickpea genotypes, which permitted to closely monitor the behavior of aphids during the inoculation process, and observe the variation of the interaction of the aphid vector with different chickpea genotypes. Six resistant (IG9000, IG69434, IG69656, IG69693, IG71832, IG128651) and 16 tolerant genotypes from different origins (Pakistan, Portugal, Spain, Morocco, India, Italy, Bulgaria, Turkey, Tunisia, Georgia, Lebanon and Syria) were identified. Highly and significant corolation (r = 0.976) was recoded between both virus infection rate (%) and disease severity with grain yield loss (%). Resistant genotypes identified can be used as a source of CpCSV resistance in chickpea breeding programs.en_US
dc.publisherArab Society for Plant Protectionen_US
dc.sourceArab Journal of Plant Protection;38,(2020) Pagination 103-114en_US
dc.subjectchickpea virusesen_US
dc.subjectresistance mechanismen_US
dc.titleDeveloping an artificial inoculation technique for screening chickpea genotypes against a Syrian isolate of Chickpea chlorotic stunt virusen_US
dc.typeJournal Articleen_US
cg.creator.idKumari, Safaa: 0000-0002-4492-6257en_US
cg.subject.agrovocgenetic resistanceen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerGeneral Commission for Scientific Agricultural Research - GCSARen_US
cg.contributor.centerUniversity of Aleppo, Faculty of Agricultureen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes and Dryland Cereals - GLDCen_US
cg.contributor.funderNSW Department of Primary Industries - NSW-DPIen_US
cg.contributor.projectNew Tools and Germplasm for Australian Pulse and Oil Seeds Breeding Programs to Respond to Changing Virus Threats (DAN00202)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionWestern Asiaen_US
cg.coverage.geolocationLatitude: 33.802538018606086; Longitude: 35.7207967939377en_US
dc.identifier.statusOpen accessen_US
cg.journalArab Journal of Plant Protectionen_US

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