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dc.contributorKumari, Safaaen_US
dc.contributorMuyldermans, Sergeen_US
dc.contributorAbbady, Abdul Qaderen_US
dc.creatorGhannam, Ahmeden_US
dc.identifier.citationAhmed Ghannam, Safaa Kumari, Serge Muyldermans, Abdul Qader Abbady. (1/3/2015). Camelid nanobodies with high affinity for broad bean mottle virus: a possible promising tool to immunomodulate plant resistance against viruses. Plant Molecular Biology, 87 (4-5), pp. 355-369.en_US
dc.description.abstractWorldwide, plant viral infections decrease seriously the crop production yield, boosting the demand to develop new strategies to control viral diseases. One of these strategies to prevent viral infections, based on the immunomodulation faces many problems related to the ectopic expression of specific antibodies in planta. Camelid nanobodies, expressed in plants, may offer a solution as they are an attractive tool to bind efficiently to viral epitopes, cryptic or not accessible to conventional antibodies. Here, we report a novel, generic approach that might lead to virus resistance based on the expression of camelid specific nanobodies against Broad bean mottle virus (BBMV). Eight nanobodies, recognizing BBMV with high specificity and affinity, were retrieved after phage display from a large 'immune' library constructed from an immunized Arabic camel. By an in vitro assay we demonstrate how three nanobodies attenuate the BBMV spreading in inoculated Vicia faba plants. Furthermore, the in planta transient expression of these three selected nanobodies confirms their virus neutralizing capacity. In conclusion, this report supports that plant resistance against viral infections can be achieved by the in vivo expression of camelid nanobodies.en_US
dc.sourcePlant Molecular Biology;87,(2015) Pagination 355-369en_US
dc.subjectvirus resistanceen_US
dc.subjectplant immunomodulationen_US
dc.subjectbroad bean mottle virusen_US
dc.subjectphage displayen_US
dc.titleCamelid nanobodies with high affinity for broad bean mottle virus: a possible promising tool to immunomodulate plant resistance against virusesen_US
dc.typeJournal Articleen_US
cg.creator.idKumari, Safaa: 0000-0002-4492-6257en_US
cg.subject.agrovocVetch (Vicia spp.)en_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerAtomic Energy Commission of Syria - AECSen_US
cg.contributor.centerVrije Universiteit Brusselen_US
cg.contributor.centerStructural Biology Research Center - VIBen_US
cg.contributor.funderAtomic Energy Commission of Syria - AECSen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusTimeless limited accessen_US
cg.journalPlant Molecular Biologyen_US

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