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dc.contributorBishop, Richard P.en_US
dc.contributorGithaka, Naftalyen_US
dc.contributorSkilton, Roberten_US
dc.contributorHeyne, Heloiseen_US
dc.contributorMwacharo, Joramen_US
dc.creatorKanduma, Esther G.en_US
dc.identifier.citationEsther G. Kanduma, Richard P. Bishop, Naftaly Githaka, Robert Skilton, Heloise Heyne, Joram Mwacharo. (1/11/2019). Mitochondrial and nuclear multilocus phylogeny of Rhipicephalus ticks from Kenya. Molecular Phylogenetics and Evolution, 140.en_US
dc.description.abstractThe morphological diversity of African ticks of the genus Rhipicephalus and subgenus Boophilus have been studied in detail. However, their taxonomy remains poorly resolved with limited molecular studies performed to improve inter-species discrimination. Herein, ribosomal cytochrome c oxidase I (COI), 12S ribosomal DNA (12S rDNA) and nuclear ribosomal DNA internal transcriber spacer 2 (ITS2) were analyzed in Rhipicephalus tick populations in Kenya. While the morphological and molecular criteria separated R. e. evertsi, R. pulchellus and R. appendiculatus from other members of the genus, except the morphologically similar sibling species R. zambeziensis, this was not the case for other tick populations. COI sequences of Rhipicephalus ticks from Ruma National Park (RNP) in Southwestern Kenya, that were morphologically similar to R. praetextatus/R. simus, a formed distinct clade and barcode gap group. 12S rDNA haplotypes of this population were 99% identical to a GenBank accession of R. muhsamae which is thought to be endemic in West and Central Africa. However, the ITS2 locus indicated that the RNP samples were genetically closest to ticks identified morphologically as R. praetextatus. The COI and 12S rDNA haplotype sequences of R. praetextatus clustered closely with R. simus reference sequences though the two species occurred in distinct barcode gap groups. Our results suggest that the R. simus/R. praetextatus/R. muhsamae comprise a closely related tick species complex found across sub-Saharan Africa and includes the yet to be described RNP population. More studies on the biology, ecology and genomics of all life stages of tick species in the complex may clarify their taxonomic status. A continent-wide study that combines morphology, DNA marker sequencing and emerging methods, such as mass spectrometry and whole-genome resequencing may reveal the diversity and distribution of taxa within the genus Rhipicephalus in sub-Saharan Africa.en_US
dc.publisherElsevier (12 months)en_US
dc.subjectgenetic diversityen_US
dc.subject12s rdnaen_US
dc.titleMitochondrial and nuclear multilocus phylogeny of Rhipicephalus ticks from Kenyaen_US
dc.typeJournal Articleen_US
cg.creator.idMwacharo, Joram: 0000-0001-6981-8140en_US
cg.subject.agrovocdna barcodingen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerInternational Livestock Research Institute - ILRIen_US
cg.contributor.centerUniversity of Nairobi - UONBIen_US
cg.contributor.centerInternational Center of Insect Physiology and Ecology - ICIPEen_US
cg.contributor.centerAgricultural Research Council South Africa - ARC South Africaen_US
cg.contributor.centerWashington State University - WSUen_US
cg.contributor.centerUniversity of Nottingham, School of Life Sciences - UoN UK - School of Life Sciencesen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionEastern Africaen_US
cg.isijournalISI Journalen_US
dc.identifier.statusTimeless limited accessen_US

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