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dc.contributorChabane, Ken_US
dc.contributorValkoun, Janen_US
dc.contributorBlake, Ten_US
dc.creatorSingh, Murarien_US
dc.identifier.citationMurari Singh, K Chabane, Jan Valkoun, T Blake. (1/2/2006). Optimum sample size for estimating gene diversity in wild wheat using AFLP markers. Genetic Resources and Crop Evolution, 53, pp. 23-33.en_US
dc.description.abstractGenetic diversity in five wild types of wheat was estimated using Simpson's index (based on heterozygosity) applied to data from AFLP markers. For such studies, the cost of obtaining the required information increases both with the number of samples required to estimate diversity and with the number of markers used. When the population studied is in Hardy-Weinberg equilibrium (HWE), allelic frequencies follow the binomial expansion and parametric methods can be used to calculate the variance of the diversity index in terms of the number of individuals sampled. Inbred species are never in HWE. With regard to such populations, this study addresses the question of the sample size required to estimate gene diversity using a distribution-free re-sampling method. We studied populations of five wild species (Aegilops speltoides, Triticum urartu, Triticum boeoticum, Triticum dicoccoides, and Triticum araraticum) as sources of diversity. We used bootstrap re-sampling with varying sample sizes to develop a relationship between the precision of the diversity estimate and the sample size. Such a relationship was used to determine the samples required for capturing a given amount of diversity and its precision. We found that 5-6 samples are sufficient to obtain a standard error equal to 10% of the diversity in the populations of the species Ae. speltoides, T. dicoccoides and T. araraticum. However, more than 12 samples would be needed for populations of T. urartu and T. boeoticum. The procedure presented here can be used to obtain the optimum sample size for other crop species as well.en_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.rightsCopyrighted; all rights reserveden_US
dc.sourceGenetic Resources and Crop Evolution;53,(2006) Pagination 23,33en_US
dc.subjectgene diversityen_US
dc.subjectamplified fragment length polymorphism (aflp)en_US
dc.subjectbootstrapping methoden_US
dc.subjecttriticum wild wheaten_US
dc.titleOptimum sample size for estimating gene diversity in wild wheat using AFLP markersen_US
dc.typeJournal Articleen_US
cg.creator.idSingh, Murari: 0000-0001-5450-0949en_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
dc.identifier.statusTimeless limited accessen_US

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