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dc.contributorBaum, Michaelen_US
dc.contributorErskine, Williamen_US
dc.contributorPehu, E.en_US
dc.contributorMuehlbauer, Fred J.en_US
dc.creatorA. Eujayl, Imaden_US
dc.identifier.citationImad A. Eujayl, Michael Baum, William Erskine, E. Pehu, Fred J. Muehlbauer. (1/8/1997). The use of Rapd markers for lentil genetic mapping and the evaluation of distorted F2 segregation. Euphytica, 96, pp. 405-412.en_US
dc.description.abstractTo maximize the extent of polymorphism within a mapping population wide crosses are often made, frequently resulting in distorted segregation. Two parents used in the crosses in this study contained ca 50% from wild lentil genome (Lens culinaris subsp. Orientalis). We investigated the use of random amplified polymorphic DNA (RAPD) in the lentil (Lens culinaris Medik), for genetic mapping and testing for segregation distortion in F-2 populations. In cross 1, 83% of the RAPD markers showed segregation distortion, which was also observed for isozyme and morphological loci. By contrast, in cross 2, there was little (10%) segregation distortion. Out of 390 primers tested, 116 primers (29.7%) yielded 192 polymorphic fragments between parents of cross 2. This polymorphism was confirmed as reproducible. Seventy-eight segregating loci were analyzed for linkage, at a LOD score > 3.0, resulted in 28 RAPD, one RFLP, one morphological and three oligonucleotide markers, which were assigned to 9 linkage groups spanning 206 cM. Clearly, in lentil RAPD markers were valuable for genetic mapping and evaluation of segregation distortion.en_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.sourceEuphytica;96,(1997) Pagination 405-412en_US
dc.subjectsegregation distortionen_US
dc.titleThe use of Rapd markers for lentil genetic mapping and the evaluation of distorted F2 segregationen_US
dc.typeJournal Articleen_US
cg.creator.idBaum, Michael: 0000-0002-8248-6088en_US
cg.subject.agrovoclens culinarisen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerUniversity of Helsinki - UoHen_US
cg.contributor.centerWashington State University - WSUen_US
cg.contributor.centerUnited States Department of Agriculture, Agricultural Research Service, Grain Legume Genetics and Physiology Research - USDA-ARS Grain Legume Genetics and Physiology Researchen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusTimeless limited accessen_US

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