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Regular updating of the crossing block with new barley varieties for conducting the crossing program of winter barley for different breeding objectives
The new sources of abiotic and biotic stresses have also been identified after evaluation through collaboration with NARS for phenotyping at diverse environments. Crossing blocks (CB) of barley 480 germplasm for spring ...
Develop MAGIC and biparental populations following SSD (2) Phenotyping of populations for target traits
Seed increase is in progress of 3000 F6 generation MAGIC lines derived from the crosses involving the parents, ICGV 91114, ICGV 06040, 55-437, ICGV 00440, ICGV 00308, GPBD 4, ICGV 05155 and ICGV 88145. RIL population derived ...
Conduct of multi-location barley international trials in target regions
Multilocation barley international trials for spring barley under low and high input environments have been conducted in Morocco, Lebanon and India. Another set of winter barley trials were conducted at Ankara, in Turkey. ...
Quantification of Foc colonization through qPCR in resistant and susceptible cultivars grown under elevated CO2
The chickpea seedlings of susceptible cv. JG 62 and resistant cv. WR 315 were grown in open top chambers at different CO2 concentrations [ambient (~380 ppm) and elevated ( 550 ppm and 700 ppm). Seven day old seedlings were ...
Use of hotspots for screening of each disease / pest
A FIGS subset of yellow rust of 298 accessions was screened in the field in Durgapura, Jaipur (India) with artificial inoculation with an inoculum consisting of a mixture of 5-pathotypes. Based on the area under disease ...
Surveys conducted to know the occurrence and distribution of diseases in chickpea and pigeonpea growing regions
For chickpea disease, a survey was conducted between November and January in four major chickpea growing states in central and southern parts of India viz. Andhra Pradesh, Karnataka and Maharashtra. For pigeonpea, survey ...
Genome wide association study (GWAS) of resistance to barley spot blotch in South Asia
Spot blotch, caused by Cochliobolus sativus (anamorph: Bipolaris Sorokiniana) is one of the important
foliar diseases of barley in South Asia. However, limited information on genetic resistance of spot blotch
resistance ...
RNA-seq based transcriptome profiling, identification and validation of heat stress responsive genes in chickpea
Six contrasting heat responsive (tolerant - ICCV 92944, ICC 1356, ICC 15614 and sensitive - ICC 5912, ICC 4567, ICC 10685) genotypes were selected for the study. Heat stress was imposed using growth chamber facility, ...
Study of expression pattern of defence genes in chickpea and pathogenicity causing genes in FOC through qPCR in resistant and susceptible cultivars grown under elevated CO2
Total RNA was extracted from root tissues of wilt infected and control chickpea seedlings of susceptible cv. JG 62 and resistant cv. WR 315 grown under ambient and elevated CO2 condition. cDNA was synthesized from total ...
Evaluation of host-plant resistance in chickpea by microbial treatment.
Three chickpea endophytes AUR-2, AUR-4 and ARR-10 were found effective against B. cinerea in vitro conditions in the previous screening. Under controlled conditions (1500 Lux light intensity, 12 h/day; 100% RH, 15±2 °C ...