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dc.contributorKiani, Sarfrazen_US
dc.contributorButt, Afshanen_US
dc.contributorQayyum Rao, Abdulen_US
dc.contributorAhmad, Aftaben_US
dc.contributorAhmad Nasir, Idreesen_US
dc.contributorHusnain, Tayyaben_US
dc.contributorMansoor, Shahiden_US
dc.contributorAmin, Imranen_US
dc.contributorAftab, Shaheenen_US
dc.contributorZubair, Muhammaden_US
dc.contributorTahir, Muhammad Noumanen_US
dc.contributorAkhtar, Sohailen_US
dc.contributorScheffler, Jodien_US
dc.contributorScheffler, Brianen_US
dc.creatorAkram, Faheemen_US
dc.identifier.citationFaheem Akram, Sarfraz Kiani, Afshan Butt, Abdul Qayyum Rao, Aftab Ahmad, Idrees Ahmad Nasir, Tayyab Husnain, Shahid Mansoor, Imran Amin, Shaheen Aftab, Muhammad Zubair, Muhammad Nouman Tahir, Sohail Akhtar, Jodi Scheffler, Brian Scheffler. (15/10/2016). Amplicon-based RNA interference targeting V2 gene of cotton leaf curl kokhran virus- burewala strain can provide resistance in transgenic cotton plants. Molecular Biotechnology, 58 (12), pp. 807-820.en_US
dc.description.abstractThe conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct targeting V2 gene of CLCuKoV-Bur, cloned under 35S promoter, was done in two elite cotton varieties MNH-786 and VH- 289 using shoot apex cut method of gene transformation. The transformation efficiency was found to be 3.75 and 2.88 % in MNH-786 and VH-289, respectively. Confirmation of successful transformation was done through PCR in T0, T1, and T2 generations using gene-specific primers. Transgenic cotton plants were categorized on the basis of the virus disease index in T1 generation. Copy number and transgene location were observed using FISH and karyotyping in T2 generation which confirmed random integration of V2 RNAi amplicon at chromosome 6 and 16. Real-time quantitative PCR analyses of promising transgenic lines showed low virus titer compared to wild-type control plants upon challenging them with viruliferous whiteflies in a contained environment. From the results, it was concluded that amplicon V2 RNAi construct was able to limit virus replication and can be used to control CLCuV in the field.en_US
dc.publisherHumana Pressen_US
dc.sourceMolecular Biotechnology;58,(2016) Pagination 807-820en_US
dc.titleAmplicon-based RNA interference targeting V2 gene of cotton leaf curl kokhran virus- burewala strain can provide resistance in transgenic cotton plantsen_US
dc.typeJournal Articleen_US
cg.subject.agrovocbread wheaten_US
cg.subject.agrovocdisease controlen_US
cg.contributor.centerUnited States Department of Agriculture, Agricultural Research Service - USDA-ARSen_US
cg.contributor.centerUniversity of Punjab, National Center of Excellence in Molecular Biology - UOP-CEMBen_US
cg.contributor.centerNational Institute for Biotechnology & Genetic Engineering - NIBGEen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderUnited States Department of Agriculture - USDAen_US
cg.contributor.projectImproving Resistance to Cotton Leaf Curl Virus (CLCuV) and Supporting Cotton Best Management Practices for Small Farmersen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionSouthern Asiaen_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
mel.funder.grant#United States Department of Agriculture - USDA :58-6402-2-167Fen_US
cg.journalMolecular Biotechnologyen_US

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