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dc.contributorLouhaichi, Mouniren_US
dc.contributorSlim, Slimen_US
dc.contributorBoulila, Abdennaceren_US
dc.contributorBettaieb, Taoufiken_US
dc.creatorHamdeni, Imtineneen_US
dc.identifier.citationImtinene Hamdeni, Mounir Louhaichi, Slim Slim, Abdennacer Boulila, Taoufik Bettaieb. (14/11/2022). Incorporation of Organic Growth Additives to Enhance In Vitro Tissue Culture for Producing Genetically Stable Plants. Plants, 11 (22).en_US
dc.description.abstractThe growing demand for native planting material in ecological restoration and rehabilitation for agro-silvo-pastoral ecosystems has resulted in a major global industry in their sourcing, multiplication, and sale. Plant tissue culture is used for producing high-quality, disease-free, and true-to-type plants at a fast rate. Micropropagation can help to meet the increasing demand for planting material and afforestation programs. However, in vitro plant propagation is an expensive technique compared to conventional methods using suckers, seeds, and cuttings. Therefore, adopting measures to lower production costs without compromising plant quality is essential. This can be achieved by improving the culture media composition. Incorporating organic growth additives can stimulate tissue growth and increase the number of shoots, leaves, and roots in culture media. Organic growth supplementation speeds up the formation and development of cultures and yields vigorous plants. Plant regeneration from meristems (shoot tips and axillary buds) is a reliable way to produce true-to-type plants compared with callus and somatic embryogenesis regeneration, but in vitro culture environments can be mutagenic. Therefore, detecting somaclonal variations at an early stage of development is considered crucial in propagating plants. The genetic stability of in vitro regenerated plants needs to be ascertained by using DNA-based molecular markers. This review aims to provide up-to-date research progress on incorporating organic growth additives to enhance in vitro tissue culture protocols and to emphasize the importance of using PCR-based molecular markers such as RAPD, ISSR, SSR, and SCoT. The review was assessed based on the peer-reviewed works published in scientific databases including Science Direct, Scopus, Springer, JSTOR, onlinelibrary, and Google Scholar.en_US
dc.subjectclimate adaptation and mitigationen_US
dc.subjectorganic growth additivesen_US
dc.subjectlarge scale restorationen_US
dc.titleIncorporation of Organic Growth Additives to Enhance In Vitro Tissue Culture for Producing Genetically Stable Plantsen_US
dc.typeJournal Articleen_US
cg.creator.idLouhaichi, Mounir: 0000-0002-4543-7631en_US
cg.creator.idSlim, Slim: 0000-0001-8294-0685en_US
cg.subject.agrovocmolecular markersen_US
cg.subject.agrovocin vitroen_US
cg.subject.agrovocplant propagationen_US
cg.subject.agrovocgoal 13 climate actionen_US
cg.subject.agrovocgenetic stabilityen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerNational Agronomic Institute of Tunisia - INATen_US
cg.contributor.centerDirection Générale des Forêts - DGFen_US
cg.contributor.centerOregon State University - OSU United Statesen_US
cg.contributor.centerEcole Supérieure d'Agriculture de Mateur - ESAMen_US
cg.contributor.centerUniversity of Carthage - UCAR Tunisen_US
cg.contributor.centerNational Institute of Research and Physico-chemical Analyses, Biotechpole of Sidi Thabeten_US
cg.contributor.crpResilient Agrifood Systems - RAFSen_US
cg.contributor.funderCGIAR System Organization - CGIARen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
cg.subject.sdgSDG 13 - Climate actionen_US
cg.subject.actionAreaResilient Agrifood Systemsen_US
cg.subject.impactAreaClimate adaptation and mitigationen_US
cg.contributor.initiativeLivestock and Climateen_US

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