Show simple item record

dc.contributorKhan, Aamiren_US
dc.contributorSaxena, Rachiten_US
dc.contributorKumar, Vinayen_US
dc.contributorKale, Sandipen_US
dc.contributorPallavi, Sinhaen_US
dc.contributorChitikineni, Annapurnaen_US
dc.contributorLekha, Pazhamalaen_US
dc.contributorVanika, Gargen_US
dc.contributorSameer Kumar, Chanda Venkataen_US
dc.contributorParupalli, Swathien_US
dc.contributorVechalapu, Suryanarayanaen_US
dc.contributorPatil, Suyashen_US
dc.contributorMuniswamy, Sonnappaen_US
dc.contributorGhanta, Anuradhaen_US
dc.contributorNarasimhan Yamini, Kalinatien_US
dc.contributorSubanna Dharmaraj, Pallavien_US
dc.contributorVarshney, Rajeeven_US
dc.creatorSingh, Vikasen_US
dc.identifier.citationVikas Singh, Aamir Khan, Rachit Saxena, Vinay Kumar, Sandip Kale, Sinha Pallavi, Annapurna Chitikineni, Pazhamala Lekha, Garg Vanika, Chanda Venkata Sameer Kumar, Swathi Parupalli, Suryanarayana Vechalapu, Suyash Patil, Sonnappa Muniswamy, Anuradha Ghanta, Kalinati Narasimhan Yamini, Pallavi Subanna Dharmaraj, Rajeev Varshney. (31/5/2016). Next-generation sequencing for identification of candidate genes for Fusarium wilt and sterility mosaic disease in pigeonpea (Cajanus cajan). Plant Biotechnology Journal, 14(5), pp. 1183-1194.en_US
dc.description.abstractTo map resistance genes for Fusarium wilt (FW) and sterility mosaic disease (SMD) in pigeonpea, sequencing-based bulked segregant analysis (Seq-BSA) was used. Resistant (R) and susceptible (S) bulks from the extreme recombinant inbred lines of ICPL 20096 9 ICPL 332 were sequenced. Subsequently, SNP index was calculated between R- and S-bulks with the help of draft genome sequence and reference-guided assembly of ICPL 20096 (resistant parent). Seq-BSA has provided seven candidate SNPs for FW and SMD resistance in pigeonpea. In parallel, four additional genotypes were re-sequenced and their combined analysis with R- and S-bulks has provided a total of 8362 nonsynonymous (ns) SNPs. Of 8362 nsSNPs, 60 were found within the 2-Mb flanking regions of seven candidate SNPs identified through Seq-BSA. Haplotype analysis narrowed down to eight nsSNPs in seven genes. These eight nsSNPs were further validated by re-sequencing 11 genotypes that are resistant and susceptible to FW and SMD. This analysis revealed association of four candidate nsSNPs in four genes with FW resistance and four candidate nsSNPs in three genes with SMD resistance. Further, In silico protein analysis and expression profiling identified two most promising candidate genes namely C.cajan_01839 for SMD resistance and C.cajan_03203 for FW resistance. Identified candidate genomic regions/ SNPs will be useful for genomics-assisted breeding in pigeonpea.en_US
dc.publisherWiley Open Accessen_US
dc.sourcePlant Biotechnology Journal;14,(2016) Pagination 1183,1194en_US
dc.subjectsterility mosaic diseaseen_US
dc.subjectbulked segregant analysisen_US
dc.subjectwhole-genome resequencingen_US
dc.subjectsnp indexen_US
dc.subjectnonsynonymous snpsen_US
dc.titleNext-generation sequencing for identification of candidate genes for Fusarium wilt and sterility mosaic disease in pigeonpea (Cajanus cajan)en_US
dc.typeJournal Articleen_US
cg.subject.agrovocfusarium wilten_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerAgricultural Research Stationen_US
cg.contributor.centerThe Professor Jayashankar Telangana State Agricultural University - PJTSAUen_US
cg.contributor.crpCRP on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.coverage.regionSouthern Asiaen_US
cg.isijournalISI journalen_US
dc.identifier.statusOpen accessen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.subject.actionAreaGenetic innovationen_US
cg.subject.actionAreaResilient agrifood systemsen_US
cg.journalPlant Biotechnology Journalen_US

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

DSpace software copyright © 2002-2016  DuraSpace
MELSpace content providers and partners accept no liability to any consequence resulting from use of the content or data made available in this repository. Users of this content assume full responsibility for compliance with all relevant national or international regulations and legislation.
Theme by 
Atmire NV