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dc.contributorMaccaferri, Marcoen_US
dc.contributorBassi, Filippoen_US
dc.contributorMantovani, Paolaen_US
dc.contributorSanguineti, Maria C.en_US
dc.contributorSalvi, Silvioen_US
dc.contributorSimkova, Hanaen_US
dc.contributorDolezel, Jaroslaven_US
dc.contributorMassi, Andreaen_US
dc.contributorAmmar, Karimen_US
dc.contributorKolmer, James Aen_US
dc.contributorTuberosa, Robertoen_US
dc.creatorTerracciano, Irmaen_US
dc.identifier.citationIrma Terracciano, Marco Maccaferri, Filippo Bassi, Paola Mantovani, Maria C. Sanguineti, Silvio Salvi, Hana Simkova, Jaroslav Dolezel, Andrea Massi, Karim Ammar, James A Kolmer, Roberto Tuberosa. (30/4/2013). Development of COS-SNP and HRM markers for highthroughput and reliable haplotype-based detection of Lr14a in durum wheat (Triticum durum Desf. ). TAG Theoretical and Applied Genetics, 126 (4), pp. 1077-1101.en_US
dc.description.abstractLeaf rust (Puccinia triticina Eriks. & Henn.) is a major disease affecting durum wheat production. The Lr14a-resistant gene present in the durum wheat cv. Creso and its derivative cv. Colosseo is one of the best characterized leaf-rust resistance sources deployed in durum wheat breeding. Lr14a has been mapped close to the simple sequence repeat markers gwm146, gwm344 and wmc10 in the distal portion of the chromosome arm 7BL, a gene-dense region. The objectives of this study were: (1) to enrich the Lr14a region with single nucleotide polymorphisms (SNPs) and high-resolution melting (HRM)-based markers developed from conserved ortholog set (COS) genes and from sequenced Diversity Array Technology (DArT®) markers; (2) to further investigate the gene content and colinearity of this region with the Brachypodium and rice genomes. Ten new COS-SNP and five HRM markers were mapped within an 8.0 cM interval spanning Lr14a. Two HRM markers pinpointed the locus in an interval of <1.0 cM and eight COS-SNPs were mapped 2.1–4.1 cM distal to Lr14a. Each marker was tested for its capacity to predict the state of Lr14a alleles (in particular, Lr14-Creso associated to resistance) in a panel of durum wheat elite germplasm including 164 accessions. Two of the most informative markers were converted into KASPar® markers. Single assay markers ubw14 and wPt-4038-HRM designed for agarose gel electrophoresis/KASPar® assays and high-resolution melting analysis, respectively, as well as the double-marker combinations ubw14/ubw18, ubw14/ubw35 and wPt-4038-HRM–ubw35 will be useful for germplasm haplotyping and for molecular-assisted breeding.en_US
dc.publisherSpringer Verlag (Germany)en_US
dc.sourceTAG Theoretical and Applied Genetics;126,(2013) Pagination 1077,1101en_US
dc.subjectleaf rusten_US
dc.subjectdurum wheaten_US
dc.subjectsimple sequence repeat markeren_US
dc.subjectchinese springen_US
dc.subjectdart markeren_US
dc.titleDevelopment of COS-SNP and HRM markers for highthroughput and reliable haplotype-based detection of Lr14a in durum wheat (Triticum durum Desf.)en_US
dc.typeJournal Articleen_US
cg.creator.idBassi, Filippo: 0000-0002-1164-5598en_US
cg.creator.idDolezel, Jaroslav: 0000-0002-6263-0492en_US
cg.contributor.centerUniversity of Bologna - UNIBOen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerInstitute of Experimental Botany - IEBen_US
cg.contributor.centerSyngenta Foundation for Sustainable Agriculture - SFSAen_US
cg.contributor.centerInternational Maize and Wheat Improvement Center - CIMMYTen_US
cg.contributor.centerUnited States Department of Agriculture, Agricultural Research Service - USDA-ARSen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
dc.identifier.statusLimited accessen_US

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