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dc.contributorSengupta, Aninditaen_US
dc.contributorNagavardhini, A.en_US
dc.contributorMamta, Sharmaen_US
dc.creatorGhosh, Rajuen_US
dc.identifier.citationRaju Ghosh, Anindita Sengupta, A. Nagavardhini, Sharma Mamta. (11/2/2015). Development of Loop-Mediated Isothermal Amplification (LAMP) assay for rapid detection of Fusarium oxysporum f. sp. ciceris - wilt pathogen of chickpea. BMC Research Notes, 8 (40), pp. 1-1.en_US
dc.description.abstractBackground Fusarium oxysporum f. sp. ciceris (Foc), the causal agent of Fusarium wilt is a devastating pathogen of chickpea. In chickpea, various soil borne pathogens produce (s) similar symptoms, therefore cannot be distinguished easily at field level. There is real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of Fusarium wilt outbreaks. Results In this study, we developed a loop-mediated isothermal amplification (LAMP) assay targeting the elongation factor 1 alpha gene sequence for visual detection of Foc. The LAMP reaction was optimal at 63°C for 60 min. When hydroxynaphthol blue (HNB) was added before amplification, samples with Foc DNA developed a characteristic sky blue colour but those without DNA or with the DNA of six other plant pathogenic fungi did not. Results obtained with LAMP and HNB were confirmed when LAMP products were subjected to gel electrophoresis. The detection limit of this LAMP assay for Foc was 10 fg of genomic DNA per reaction, while that of conventional PCR was 100 pg. Conclusions In conclusion, it was found that a LAMP assay combined with HNB is simple, rapid, sensitive, and specific. The LAMP assay does not require specialized equipment, hence can be used in the field for the rapid detection of Foc. This is the first report of the use of LAMP assay for the detection of Foc. The presented LAMP method provides a specific, sensitive and rapid diagnostic tool for the distinction of Foc, with the potential to be standardized as a detection method for Foc in endemic areas and will be very useful for monitoring the disease complex in the field further suggesting the management strategies.en_US
dc.publisherBioMed Centralen_US
dc.sourceBMC Research Notes;8,(2015) Pagination 1,1en_US
dc.subjecthydroxynaphthol blueen_US
dc.subjectisothermal amplificationen_US
dc.titleDevelopment of Loop-Mediated Isothermal Amplification (LAMP) assay for rapid detection of Fusarium oxysporum f. sp. ciceris - wilt pathogen of chickpeaen_US
dc.typeJournal Articleen_US
cg.creator.idMamta, Sharma: 0000-0001-5745-4693en_US
cg.subject.agrovocpest managementen_US
cg.subject.agrovocfusarium oxysporumen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.crpCRP on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
dc.identifier.statusOpen accessen_US

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