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dc.creatorAyed, Sourouren_US
dc.identifier.citationSourour Ayed. (31/3/2014). Efficient Production of Tetraploid Barley (Hordeum vulgare L. ) by Colchicine Treatment of Diploid Barley. Journal of Experimental Biology and Agricultural Sciences, 2.en_US
dc.description.abstractAn experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments. Colchicine was added for three different concentrations at three different stages of plant development i.e. on seed (0.05% for 48 hours), on pre-germinated seeds (0.1% for 2 hours) and on three leaf stage (0.1% for 16 hours). Colchicine application reduced significantly germination percentage and viability of plants. Seed germination was completely inhibited in Martin, while a reduction of 20% and 30% for germination percentage compared to control was recorded in varieties Manel and Rihane, respectively at 0.1% colchicine concentration. Ploidy evaluation showed no tetraploidy in all the three tested varieties by colchicine application of 0.05% for 48 hours on seeds and 0.1% for 2 hours on pre-germinated seeds. However, tetraploid plants were produced only by treatment with 0.1% for 16 hours of seedlings. The percentages of plants were 40%, 44% and 100% for Rihane, Manel and Martin, respectively. Cytological analyses showed the increase of chromosome numbers from 2n=2x=14 to 2n=4x=28. The increase of ploidy levels caused major changes in some morphological traits. In fact, the induced tetraploids in barley was accompanied by significant (P<0.01) decrease in plant height, tiller height, leaf number and leaf length compared to diploid control plants. colchicine treatment induce successfully the production of tetraploid barley plants and could be used in breeding programs.en_US
dc.publisherHorizon Publisher India (HPI)en_US
dc.sourceJournal of Experimental Biology and Agricultural Sciences;2,(2014)en_US
dc.subjectefficient productionen_US
dc.subjecttetraploid barleyen_US
dc.subjectdiploid barleyen_US
dc.subjectcolchicine treatmenten_US
dc.titleEfficient Production of Tetraploid Barley (Hordeum vulgare L.) by Colchicine Treatment of Diploid Barleyen_US
dc.typeJournal Articleen_US
cg.contributor.centerEcole Supérieure d’Agriculture du Kef - ESA Kefen_US
cg.contributor.crpCGIAR Research Program on Dryland Systems - DSen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderInternational Fund for Agricultural Development - IFADen_US
cg.contributor.projectEnhanced small-holder wheat-legume cropping systems to improve food security under changing climate in the drylands of West Asia and North Africaen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionNorthern Africaen_US
dc.identifier.statusOpen accessen_US
cg.journalJournal of Experimental Biology and Agricultural Sciencesen_US

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