Effect of genotypes and culture media on embryogenic callus induction and plantlet regeneration from mature embryos of durum wheat

cg.contacts.udupa@cgiar.orgen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerNational Institute of Agronomic Research Morocco - INRA Moroccoen_US
cg.contributor.crpCGIAR Research Program for Managing and Sustaining Crop Collections - Genebanksen_US
cg.contributor.funderGovernment of Morocco - Moroccoen_US
cg.contributor.projectMoroccan Collaborative Grants Program (MCGP)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.creator.idUdupa, Sripada M.: 0000-0003-4225-7843en_US
cg.isijournalISI Journalen_US
cg.journalRomanian Agricultural Researchen_US
cg.subject.agrovocgenetic resourcesen_US
cg.subject.agrovocplant genetic resourcesen_US
cg.subject.agrovocWheaten_US
cg.volume31en_US
dc.contributorUdupa, Sripada M.en_US
dc.contributorAbdelwahd, Rabhaen_US
dc.creatorHakam, Najaten_US
dc.date.accessioned2017-02-09T16:34:31Z
dc.date.available2017-02-09T16:34:31Z
dc.description.abstractIn wheat, immature embryos are the most widely used explant to initiate cultures, but they are inconvenient due to their temporal availability and production requirements. Mature embryos are easily stored and are readily available as mature seeds. However, plant regeneration frequencies from cultures derived from mature embryos are generally low. This study was undertaken to improve callus induction and plant regeneration from durum wheat (Triticum turgidum L. var. durum) mature embryos for biolistic and Agrobacterium-mediated transformation. Five wheat genotypes were evaluated for their response to callus induction and regeneration on M3 medium (M3a, M3b, M3c, M3d, M3e and M3f) modified with different concentrations of 2,4 Dichlorophenoxyacetic acid (2,4-D), that is, 1mg/L and 2mg/L, vitamins, Maltose and sucrose, which gives good results both in the callus induction and in the regeneration of plantlets. A significant effect of variety, medium and variety x medium interaction were observed for callus induction and regeneration. Relative fresh weight growth rate (RFWGR), calculated after 4 weeks of incubation of the explants across six media, was highest for Chaoui (8189.2%), and the lowest for Kyperounda and Marouane (4750.3% and 4027.7%). M3e recorded the highest (6923.9%) followed by M3d (6424.2%), M3f (5971.4%) and M3c (5663.3%), and M3a (5275.4%), whereas, M3b was the lowest (3950.4%). M3e, M3c, M3d and M3f showed higher RFWGR for callus after 8 weeks of culture, M3f medium showed the highest percentage of plantlet regeneration (54.80%), and all of the other media they gave similar results. In this study favorable media, which gave good results both in the callus induction and in the regeneration of plantlets, were identified and will be used for callus induction and genetic transformation.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/NoRiov7n/v/609966d63c87accd0dd36151f391cc08en_US
dc.identifier.citationNajat Hakam, Sripada M. Udupa, Rabha Abdelwahd. (20/6/2014). Effect of genotypes and culture media on embryogenic callus induction and plantlet regeneration from mature embryos of durum wheat. ROMANIAN AGRICULTURAL RESEARCH, 31.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/5648
dc.languageenen_US
dc.publisherNational Agricultural Research and Development Institute (NARDI Fundulea)en_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceRomanian Agricultural Research;31,(2014)en_US
dc.subjectgeneticen_US
dc.titleEffect of genotypes and culture media on embryogenic callus induction and plantlet regeneration from mature embryos of durum wheaten_US
dc.typeJournal Articleen_US
dcterms.available2014-06-20en_US
mel.impact-factor0.36en_US
mel.project.openhttps://mel.cgiar.org/projects/195en_US

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