Transcriptome analyses reveal genotype- and developmental stage-specific molecular responses to drought and salinity stresses in chickpea

cg.contactmjain@nipgr.ac.inen_US
cg.contributor.centerNational Institute of Plant Genome Research - NIPGRen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerRMIT University, School of Science - RMIT - Scienceen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.coverage.countryINen_US
cg.coverage.regionSouthern Asiaen_US
cg.identifier.doihttps://dx.doi.org/10.1038/srep19228en_US
cg.isijournalISI Journalen_US
cg.issn2045-2322en_US
cg.journalScientific Reportsen_US
cg.subject.agrovocdroughten_US
cg.subject.agrovocgenomicsen_US
cg.subject.agrovocgenotypesen_US
cg.volume6 (19228)en_US
dc.contributorShankar, Ramaen_US
dc.contributorThakkar, Bijalen_US
dc.contributorKudapa, Hima binduen_US
dc.contributorKrishnamurty, Lakshmananen_US
dc.contributorMantri, Nitinen_US
dc.contributorVarshney, Rajeeven_US
dc.contributorBhatia, Sabhyataen_US
dc.contributorJain, Mukeshen_US
dc.creatorGarg, Rohinien_US
dc.date.accessioned2017-04-11T22:34:02Z
dc.date.available2017-04-11T22:34:02Z
dc.description.abstractDrought and salinity are the major factors that limit chickpea production worldwide. We performed whole transcriptome analyses of chickpea genotypes to investigate the molecular basis of drought and salinity stress response/adaptation. Phenotypic analyses confirmed the contrasting responses of the chickpea genotypes to drought or salinity stress. RNA-seq of the roots of drought and salinity related genotypes was carried out under control and stress conditions at vegetative and/or reproductive stages. Comparative analysis of the transcriptomes revealed divergent gene expression in the chickpea genotypes at different developmental stages. We identified a total of 4954 and 5545 genes exclusively regulated in drought-tolerant and salinity-tolerant genotypes, respectively. A significant fraction (~47%) of the transcription factor encoding genes showed differential expression under stress. The key enzymes involved in metabolic pathways, such as carbohydrate metabolism, photosynthesis, lipid metabolism, generation of precursor metabolites/energy, protein modification, redox homeostasis and cell wall component biogenesis, were affected by drought and/or salinity stresses. Interestingly, transcript isoforms showed expression specificity across the chickpea genotypes and/or developmental stages as illustrated by the AP2-EREBP family members. Our findings provide insights into the transcriptome dynamics and components of regulatory network associated with drought and salinity stress responses in chickpeaen_US
dc.formatPDFen_US
dc.identifierhttp://oar.icrisat.org/id/eprint/9276en_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/KXAEpUHX/v/40244d8a6f279ba520f976399ba56060en_US
dc.identifier.citationRohini Garg, Rama Shankar, Bijal Thakkar, Hima bindu Kudapa, Lakshmanan Krishnamurty, Nitin Mantri, Rajeev Varshney, Sabhyata Bhatia, Mukesh Jain. (13/1/2016). Transcriptome analyses reveal genotype- and developmental stage-specific molecular responses to drought and salinity stresses in chickpea. Scientific Reports, 6 (19228), pp. 1-15.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6718
dc.languageenen_US
dc.publisherNature Publishing Group:en_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceScientific Reports;6 (19228),(2016) Pagination 1,15en_US
dc.subjectmolecular responsesen_US
dc.subjectsalinity stressesen_US
dc.subjectchickpea productionen_US
dc.subjectChickpeaen_US
dc.subjectLegumeen_US
dc.titleTranscriptome analyses reveal genotype- and developmental stage-specific molecular responses to drought and salinity stresses in chickpeaen_US
dc.typeJournal Articleen_US
dcterms.available2016-01-13en_US
dcterms.extent1-15en_US
mel.impact-factor5.228en_US

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