Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes
cg.contact | P.Janila@cgiar.org | en_US |
cg.contributor.center | International Crops Research Institute for the Semi-Arid Tropics - ICRISAT | en_US |
cg.contributor.center | Indian Council of Agricultural Research, Directorate of Groundnut Research - ICAR - DGR | en_US |
cg.contributor.crp | CGIAR Research Program on Grain Legumes - GL | en_US |
cg.contributor.funder | Not Applicable | en_US |
cg.coverage.region | Global | en_US |
cg.creator.id | Janila, Pasupuleti: 0000-0003-2583-9630 | en_US |
cg.creator.id | Murali, TV: 0000-0001-5775-0329 | en_US |
cg.identifier.doi | https://dx.doi.org/10.1016/j.plantsci.2015.08.013 | en_US |
cg.isijournal | ISI Journal | en_US |
cg.journal | Plant Science | en_US |
cg.subject.agrovoc | plant breeding | en_US |
cg.subject.agrovoc | plant genetic resources | en_US |
cg.subject.agrovoc | oil content | en_US |
cg.subject.agrovoc | oleic acid | en_US |
cg.subject.agrovoc | peanuts | en_US |
cg.subject.agrovoc | marker-assisted selection | en_US |
cg.volume | 242 | en_US |
dc.contributor | Mishra, Gyan | en_US |
dc.contributor | Khera, Pawan | en_US |
dc.contributor | Manohar, Surendra | en_US |
dc.contributor | Patne, Nagesh | en_US |
dc.contributor | Shasidhar, Yaduru | en_US |
dc.contributor | Sriswathi, Manda | en_US |
dc.contributor | Vishwakarma, Manish K. | en_US |
dc.contributor | Murali, TV | en_US |
dc.creator | Janila, Pasupuleti | en_US |
dc.date.accessioned | 2017-08-16T12:06:25Z | |
dc.date.available | 2017-08-16T12:06:25Z | |
dc.description.abstract | High oleate peanuts have two marketable benefits, health benefits to consumers and extended shelf life of peanut products. Two mutant alleles present on linkage group a09 (ahFAD2A) and b09 (ahFAD2B) control composition of three major fatty acids, oleic, linoleic and palmitic acids which together determine peanut oil quality. In conventional breeding, selection for fatty acid composition is delayed to advanced generations. However by using DNA markers, breeders can reject large number of plants in early generations and therefore can optimize time and resources. Here, two approaches of molecular breeding namely marker-assisted backcrossing (MABC) and marker-assisted selection (MAS) were employed to transfer two FAD2 mutant alleles from SunOleic 95R into the genetic background of ICGV 06110, ICGV 06142 and ICGV 06420. In summary, 82 MABC and 387 MAS derived introgression lines (ILs) were developed using DNA markers with elevated oleic acid varying from 62 to 83%. Oleic acid increased by 0.5–1.1 folds, with concomitant reduction of linoleic acid by 0.4–1.0 folds and palmitic acid by 0.1–0.6 folds among ILs compared to recurrent parents. Finally, high oleate ILs, 27 with high oil (53–58%), and 28 ILs with low oil content (42–50%) were selected that may be released for cultivation upon further evaluation. | en_US |
dc.format | en_US | |
dc.identifier | http://www.sciencedirect.com/science/article/pii/S0168945215300455 | en_US |
dc.identifier | https://mel.cgiar.org/reporting/downloadmelspace/hash/THueIwQL/v/cfa20da621f146c096eb520104a18cea | en_US |
dc.identifier.citation | Pasupuleti Janila, Gyan Mishra, Pawan Khera, Surendra Manohar, Nagesh Patne, Yaduru Shasidhar, Manda Sriswathi, Manish K. Vishwakarma, TV Murali. (31/12/2015). Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes. Plant Science, 242, pp. 203-2013. | en_US |
dc.identifier.status | Open access | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/7436 | |
dc.language | en | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | CC-BY-NC-4.0 | en_US |
dc.source | Plant Science;242,(2015) Pagination 203,2013 | en_US |
dc.subject | oil quality | en_US |
dc.subject | marker-assisted backcrossing | en_US |
dc.subject | Peanut | en_US |
dc.title | Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes | en_US |
dc.type | Journal Article | en_US |
dcterms.available | 2015-12-31 | en_US |
dcterms.extent | 203-2013 | en_US |
mel.impact-factor | 3.362 | en_US |