Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys

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cg.contactr.k.varshney@cgiar.orgen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerLeibniz Institute of Plant Genetics and Crop Plant Research - ipk-gaterslebenen_US
cg.contributor.centerCenter for Cellular and Molecular Biology - CCMBen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryAFen_US
cg.coverage.regionSouthern Asiaen_US
cg.date.embargo-end-dateTimelessen_US
cg.identifier.doihttps://dx.doi.org/10.1016/j.plantsci.2007.08.010en_US
cg.isijournalISI Journalen_US
cg.issn0168-9452en_US
cg.issue6en_US
cg.journalPlant Scienceen_US
cg.subject.agrovocbarleyen_US
cg.subject.agrovocmolecular markersen_US
cg.subject.agrovocsnpen_US
cg.subject.agrovocaflpen_US
cg.subject.agrovocssren_US
cg.subject.agrovocBarleyen_US
cg.volume173en_US
dc.contributorChabane, Kamelen_US
dc.contributorHendre, Prasaden_US
dc.contributorAggarwal, Rameshen_US
dc.contributorGraner, Andreasen_US
dc.creatorVarshney, Rajeeven_US
dc.date.accessioned2020-10-28T22:17:18Z
dc.date.available2020-10-28T22:17:18Z
dc.description.abstractA set of 16 expressed sequence tag (EST)-derived simple sequence repeat (SSR) and 15 EST-derived single nucleotide polymorphism (SNP) markers together with 4 amplified fragment length polymorphism (AFLP) primer combinations were analyzed on 43 wild (Hordeum vulgare ssp. spontaneum - HS), 35 cultivated (H. vulgare ssp. vulgare - HV) and 12 elite (H. vulgare ssp. vulgare - from EU) barley lines. SSR markers were found most polymorphic with an average PIC value of 0.593 and eight alleles per marker, while AFLP markers showed the highest effective multiplex ratio (26.4) and marker index (5.042). The effective marker index (EMI) was recorded highest (0.468) for AFLP markers and lowest (0.341) for the SNP markers while the SSR markers had an intermediate EMI (0.442). Cluster analysis on combined set of SSR, SNP and AFLP genotyping data classified wild, cultivated and elite barley lines in three distinct groups. The present study suggests the SNP markers as the best class of markers for characterizing and conserving the genebank materials and the AFLP and SSR markers more suitable for diversity analysis and fingerprinting. (c) 2007 Elsevier Ireland Ltd. All rights reserved.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifier.citationRajeev Varshney, Kamel Chabane, Prasad Hendre, Ramesh Aggarwal, Andreas Graner. (1/12/2007). Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant Science, 173 (6), pp. 638-649.en_US
dc.identifier.statusTimeless limited accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/11993
dc.languageenen_US
dc.publisherElsevieren_US
dc.sourcePlant Science;173,(2007) Pagination 638-649en_US
dc.subjectgenetic diversityen_US
dc.titleComparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleysen_US
dc.typeJournal Articleen_US
dcterms.available2007-09-04en_US
dcterms.extent638-649en_US
dcterms.issued2007-12-01en_US
mel.impact-factor3.591en_US

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