First Molecular Identification and Genetic Characterization of Theileria lestoquardi in Sheep of the Maghreb Region

cg.contactmedridharjeibi@yahoo.fren_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerThe Institution of Research and Higher Agricultural Education - IRESAen_US
cg.contributor.centerManouba University, National School of Veterinary Medicine of Sidi Thabet - ENMVen_US
cg.contributor.crpCGIAR Research Program on Dryland Systems - DSen_US
cg.contributor.funderCGIAR System Organization - CGIARen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryTNen_US
cg.coverage.regionNorthern Africaen_US
cg.creator.idRekik, Mourad: 0000-0001-7455-2017en_US
cg.date.embargo-end-dateTimelessen_US
cg.identifier.doihttps://dx.doi.org/10.1111/tbed.12271en_US
cg.isijournalISI Journalen_US
cg.issn1865-1674en_US
cg.issue3en_US
cg.journalTransboundary and Emerging Diseasesen_US
cg.subject.agrovocagricultureen_US
cg.subject.agrovoclivestocken_US
cg.subject.agrovoctunisiaen_US
cg.subject.agrovocpcren_US
cg.subject.agrovocmaghreben_US
cg.volume63en_US
dc.contributorDarghouth, Mohamed Azizen_US
dc.contributorRekik, Mouraden_US
dc.contributorAmor, B.en_US
dc.contributorSassi, L.en_US
dc.contributorGharbi, Mohameden_US
dc.creatorRijeibi, Mohammeden_US
dc.date.accessioned2017-03-14T21:53:21Z
dc.date.available2017-03-14T21:53:21Z
dc.description.abstractTheileria lestoquardi is the most prominent Theileria species in small ruminants that causes malignant theileriosis of sheep in Africa and Asia. In the present survey, blood samples and ticks were collected in Kebili (southern Tunisia) from 166 Queue Fine de l’Ouest sheep. Giemsa-stained blood smears, immunofluorescent antibody test (IFAT) and PCR were performed. The DNA was extracted from blood and analysed by PCR targeting 18S rRNA gene of Theileria spp. and then sequenced. A total number of 140 ticks were collected from a total number of 166 sheep during the four seasons. The ticks belonged to two genera and 4 species; the most frequent tick was Hyalomma excavatum 84.3% (118/140) and then Rhipicephalus spp. 15.7% (22/140). Only two animals had positive Giemsa-stained blood smears, and they were also positive by IFAT. The amplicons had 99.3 and 99.6% homology with the BLAST published T. lestoquardi amplicons. To our knowledge, this is the first report of T. lestoquardi in small ruminants within the Maghreb region.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifierhttps://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.12271en_US
dc.identifier.citationMohammed Rijeibi, Mohamed Aziz Darghouth, Mourad Rekik, B. Amor, L. Sassi, Mohamed Gharbi. (28/6/2016). First Molecular Identification and Genetic Characterization of Theileria lestoquardi in Sheep of the Maghreb Region. Transboundary and Emerging Diseases, 63 (3), pp. 278-284.en_US
dc.identifier.statusTimeless limited accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6481
dc.languageenen_US
dc.publisherWileyen_US
dc.sourceTransboundary and Emerging Diseases;63,(2014) Pagination 278-284en_US
dc.subjectheepen_US
dc.subjecttheileria lestoquardien_US
dc.titleFirst Molecular Identification and Genetic Characterization of Theileria lestoquardi in Sheep of the Maghreb Regionen_US
dc.typeJournal Articleen_US
dcterms.available2014-09-11en_US
dcterms.extent278-284en_US
dcterms.issued2016-06-28en_US
mel.impact-factor3.504en_US

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