First Molecular Identification and Genetic Characterization of Theileria lestoquardi in Sheep of the Maghreb Region
cg.contact | medridharjeibi@yahoo.fr | en_US |
cg.contributor.center | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.contributor.center | The Institution of Research and Higher Agricultural Education - IRESA | en_US |
cg.contributor.center | Manouba University, National School of Veterinary Medicine of Sidi Thabet - ENMV | en_US |
cg.contributor.crp | CGIAR Research Program on Dryland Systems - DS | en_US |
cg.contributor.funder | CGIAR System Organization - CGIAR | en_US |
cg.contributor.project-lead-institute | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.coverage.country | TN | en_US |
cg.coverage.region | Northern Africa | en_US |
cg.creator.id | Rekik, Mourad: 0000-0001-7455-2017 | en_US |
cg.date.embargo-end-date | Timeless | en_US |
cg.identifier.doi | https://dx.doi.org/10.1111/tbed.12271 | en_US |
cg.isijournal | ISI Journal | en_US |
cg.issn | 1865-1674 | en_US |
cg.issue | 3 | en_US |
cg.journal | Transboundary and Emerging Diseases | en_US |
cg.subject.agrovoc | agriculture | en_US |
cg.subject.agrovoc | livestock | en_US |
cg.subject.agrovoc | tunisia | en_US |
cg.subject.agrovoc | pcr | en_US |
cg.subject.agrovoc | maghreb | en_US |
cg.volume | 63 | en_US |
dc.contributor | Darghouth, Mohamed Aziz | en_US |
dc.contributor | Rekik, Mourad | en_US |
dc.contributor | Amor, B. | en_US |
dc.contributor | Sassi, L. | en_US |
dc.contributor | Gharbi, Mohamed | en_US |
dc.creator | Rijeibi, Mohammed | en_US |
dc.date.accessioned | 2017-03-14T21:53:21Z | |
dc.date.available | 2017-03-14T21:53:21Z | |
dc.description.abstract | Theileria lestoquardi is the most prominent Theileria species in small ruminants that causes malignant theileriosis of sheep in Africa and Asia. In the present survey, blood samples and ticks were collected in Kebili (southern Tunisia) from 166 Queue Fine de l’Ouest sheep. Giemsa-stained blood smears, immunofluorescent antibody test (IFAT) and PCR were performed. The DNA was extracted from blood and analysed by PCR targeting 18S rRNA gene of Theileria spp. and then sequenced. A total number of 140 ticks were collected from a total number of 166 sheep during the four seasons. The ticks belonged to two genera and 4 species; the most frequent tick was Hyalomma excavatum 84.3% (118/140) and then Rhipicephalus spp. 15.7% (22/140). Only two animals had positive Giemsa-stained blood smears, and they were also positive by IFAT. The amplicons had 99.3 and 99.6% homology with the BLAST published T. lestoquardi amplicons. To our knowledge, this is the first report of T. lestoquardi in small ruminants within the Maghreb region. | en_US |
dc.format | en_US | |
dc.identifier | https://mel.cgiar.org/dspace/limited | en_US |
dc.identifier | https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.12271 | en_US |
dc.identifier.citation | Mohammed Rijeibi, Mohamed Aziz Darghouth, Mourad Rekik, B. Amor, L. Sassi, Mohamed Gharbi. (28/6/2016). First Molecular Identification and Genetic Characterization of Theileria lestoquardi in Sheep of the Maghreb Region. Transboundary and Emerging Diseases, 63 (3), pp. 278-284. | en_US |
dc.identifier.status | Timeless limited access | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/6481 | |
dc.language | en | en_US |
dc.publisher | Wiley | en_US |
dc.source | Transboundary and Emerging Diseases;63,(2014) Pagination 278-284 | en_US |
dc.subject | heep | en_US |
dc.subject | theileria lestoquardi | en_US |
dc.title | First Molecular Identification and Genetic Characterization of Theileria lestoquardi in Sheep of the Maghreb Region | en_US |
dc.type | Journal Article | en_US |
dcterms.available | 2014-09-11 | en_US |
dcterms.extent | 278-284 | en_US |
dcterms.issued | 2016-06-28 | en_US |
mel.impact-factor | 3.504 | en_US |