Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus

cg.contactshahidmansoor7@gmail.comen_US
cg.contributor.centerNational Institute for Biotechnology & Genetic Engineering - NIBGEen_US
cg.contributor.crpCRP on Wheat - WHEATen_US
cg.contributor.funderUnited States Department of Agriculture - USDAen_US
cg.contributor.projectImproving Resistance to Cotton Leaf Curl Virus (CLCuV) and Supporting Cotton Best Management Practices for Small Farmersen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryPKen_US
cg.coverage.regionSouthern Asiaen_US
cg.isijournalISI Journalen_US
cg.journalVirology Journalen_US
cg.subject.agrovocagricultureen_US
cg.subject.agrovocbiotechnologyen_US
cg.subject.agrovocdisease controlen_US
cg.volume10en_US
dc.contributorAmin, Imranen_US
dc.contributorBriddon, Rob W.en_US
dc.contributorMansoor, Shahiden_US
dc.creatorAli, Irfanen_US
dc.date.accessioned2017-01-19T16:35:59Z
dc.date.available2017-01-19T16:35:59Z
dc.description.abstractBackground: Cotton leaf curl disease, caused by single-stranded DNA viruses of the genus Begomovirus (family Geminiviridae), is a major constraint to cotton cultivation across Pakistan and north-western India. At this time only cotton varieties with moderate tolerance are available to counter the disease. microRNAs (miRNAs) are a class of endogenous small RNA molecules that play an important role in plant development, signal transduction, and response to biotic and a biotic stress. Studies have shown that miRNAs can be engineered to alter their target specificity. Such artificial miRNAs (amiRNAs) have been shown to provide resistance against plant-infecting viruses. Results: Two amiRNA constructs, based on the sequence of cotton miRNA169a, were produced containing 21 nt of the V2 gene sequence of Cotton leaf curl Burewala virus (CLCuBuV) and transformed into Nicotiana benthamiana. The first amiRNA construct (P1C) maintained the miR169a sequence with the exception of the replaced 21 nt whereas in the second (P1D) the sequence of the miRNA169a backbone was altered to restore some of the hydrogen bonding of the mature miRNA duplex. P1C transgenic plants showed good resistance when challenge with CLCuBV; plants being asymptomatic with low viral DNA levels. The resistance to heterologous viruses was lower and correlated with the numbers of sequence mismatches between the amiRNA and the V2 gene sequence. P1D plants showed overall poorer resistance to challenge with all viruses tested. Conclusions: The results show that the amiRNA approach can deliver efficient resistance in plants against a monopartite begomoviruses and that this has the potential to be broad-spectrum, providing protection from a number of viruses. Additionally the findings indicate that the levels of resistance depend upon the levels of complementarity between the amiRNA and the target sequence and the sequence of the miRNA backbone, consistent with earlier studies.en_US
dc.formatPDFen_US
dc.identifierhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765727/pdf/1743-422X-10-231.pdfen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/lZ9Ed6ze/v/c321eb127fdafedc3e764f67466a3bbcen_US
dc.identifier.citationIrfan Ali, Imran Amin, Rob W. Briddon, Shahid Mansoor. (12/7/2013). Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus. Virology Journal, 10, pp. 231-239.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/5527
dc.languageenen_US
dc.publisherBio Med Centralen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceVirology Journal;10,(2013) Pagination 231,239en_US
dc.subjectWheaten_US
dc.titleArtificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virusen_US
dc.typeJournal Articleen_US
dcterms.available2013-07-12en_US
dcterms.extent231-239en_US
mel.impact-factor5.076en_US
mel.project.openhttps://www.ars.usda.gov/research/project/?accnNo=423629en_US

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