Molecular identification of chlamydial cause of abortion in small ruminants in Jordan
Views
0% 0
Downloads
0 0%
Limited access
Citation
Hutheifa Ababneh, Mustafa Ababneh, Wael M. Hananeh, Fawzi Al-Sheyab, Khaleel I. Jawasreh, Moath Al-Gharaibeh, Mohammed Ababneh. (12/8/2014). Molecular identification of chlamydial cause of abortion in small ruminants in Jordan. Tropical animal health and production, 46(8), pp. 1407-1412.
Abstract
Chlamydophila abortus (Ch. abortus) is the etiological
agent of ovine enzootic abortion (OEA) and one of the
most common infectious agents of abortion in small ruminants
worldwide. RFLP-PCR analysis of the outer membrane protein
gene (OMP2 gene) was used for diagnosis and characterization
of chlamydial causes of abortion in small ruminants in
Jordan. Sixty-six placental tissues and 15 vaginal swabs were
collected from aborted ewes and does to identify cause of
abortion in Jordan. Thirty-eight placental samples (58 %) and
13 vaginal swabs (87 %) were positive for chlamydial DNA.
Shedding of bacteria in vaginal swabs was detected within
7 days after abortion. The results of this study showed that
chlamydiosis is one of the important causes of abortion in
small ruminants in Jordan. In addition, vaginal swab is an
excellent sample for molecular diagnosis of chlamydiosis.
DNA sequencing and RFLP analysis of the OMP2 reveal that
all chlamydial cause of abortion in small ruminants in Jordan
are due to Ch. abortus. While, Ch. pecorum was not detected
in any sample. OMP2 gene of the isolated Jordanian strain
was identical (100 %) to Ch. abortus FAS strain. In conclusion,
Ch. abortus is an important cause of abortion in Jordan;
vaginal swab within 7 days of abortion can be used for
molecular diagnosis of chlamydiosis in small ruminants.
