Tryptophan fluorescence quenching as a binding assay to monitor protein conformation changes in the membrane of intact mitochondria
cg.contact | H.SHARMA@CGIAR.ORG | en_US |
cg.contributor.center | International Crops Research Institute for the Semi-Arid Tropics - ICRISAT | en_US |
cg.contributor.center | Gulbarga University | en_US |
cg.contributor.crp | CGIAR Research Program on Grain Legumes - GL | en_US |
cg.contributor.funder | Not Applicable | en_US |
cg.coverage.country | IN | en_US |
cg.coverage.region | Southern Asia | en_US |
cg.date.embargo-end-date | 2017-06-30 | en_US |
cg.identifier.doi | https://dx.doi.org/10.1007/s10863-016-9653-0 | en_US |
cg.isijournal | ISI Journal | en_US |
cg.issn | 1573-6881 | en_US |
cg.issue | 3 | en_US |
cg.journal | Journal of Bioenergetics and Biomembranes | en_US |
cg.subject.agrovoc | agriculture | en_US |
cg.subject.agrovoc | mitochondria | en_US |
cg.subject.agrovoc | cytochrome c | en_US |
cg.volume | 48 | en_US |
dc.contributor | Sreeramulu, Kuruba | en_US |
dc.contributor | Sharma, Hari C. | en_US |
dc.creator | Akbar, Mohammad S. | en_US |
dc.date.accessioned | 2017-04-08T22:15:23Z | |
dc.date.available | 2017-04-08T22:15:23Z | |
dc.description.abstract | Intrinsic protein fluorescence is due to aromatic amino acids, mainly tryptophan, which can be selectively measured by exciting at 295 nm. Changes in emission spectra of tryptophan are due to the protein conformational transitions, subunit association, ligand binding or denaturation, which affect the local environment surrounding the indole ring. In this study, tryptophan fluorescence was monitored in intact mitochondria at 333 nm following excitation at 295 nm in presence of insecticides using spectrofluorometer. Methylparathion, carbofuran, and endosulfan induced Trp fluorescence quenching and release of cytochrome c when incubated with the mitochondria, except fenvalarate. Mechanism of insecticide-induced mitochondrial toxicity for the tested insecticides has been discussed. Reduction in the intensity of tryptophan emission spectra of mitochondrial membrane proteins in presence of an increasing concentration of a ligand can be used to study the interaction of insecticides/drugs with the intact mitochondria. Furthermore, this assay can be readily adapted for studying protein–ligand interactions in intact mitochondria and in other cell organelles extending its implications for pesticide and pharma industry and in drug discovery. | en_US |
dc.format | en_US | |
dc.identifier | http://oar.icrisat.org/id/eprint/9365 | en_US |
dc.identifier | https://mel.cgiar.org/reporting/downloadmelspace/hash/fHZTAaTB/v/1277e2ee2eb6db9fc4c11b337632c620 | en_US |
dc.identifier.citation | Mohammad S. Akbar, Kuruba Sreeramulu, Hari C. Sharma. (30/6/2016). Tryptophan fluorescence quenching as a binding assay to monitor protein conformation changes in the membrane of intact mitochondria. Journal of Bioenergetics and Biomembranes, 48 (3), pp. 241-7. | en_US |
dc.identifier.status | Limited access | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/6660 | |
dc.language | en | en_US |
dc.publisher | Springer Verlag (Germany) | en_US |
dc.rights | CC-BY-NC-4.0 | en_US |
dc.source | Journal of Bioenergetics and Biomembranes;48,(2016) Pagination 241,7 | en_US |
dc.subject | trp fluorescence quenching | en_US |
dc.subject | insecticide | en_US |
dc.title | Tryptophan fluorescence quenching as a binding assay to monitor protein conformation changes in the membrane of intact mitochondria | en_US |
dc.type | Journal Article | en_US |
dcterms.available | 2016-06-30 | en_US |
dcterms.extent | 241-7 | en_US |
mel.impact-factor | 2.080 | en_US |