Radiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheat

cg.contactShahryar.Kianian@usda.goven_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerAustralian Center for International Agricultural Research - ACIARen_US
cg.contributor.centerDiversity Arrays Technology Pty Ltd - DArTen_US
cg.contributor.centerUnited States Department of Agriculture, Agricultural Research Service - USDA-ARSen_US
cg.contributor.centerNorth Dakota State University - NDSUen_US
cg.contributor.centerFrench National Research Institute for Agriculture, Food and Environment, Genetics, Diversity and Ecophysiology of Cereals - INRAE -UMR GDECen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.creator.idBassi, Filippo: 0000-0002-1164-5598en_US
cg.identifier.doihttps://dx.doi.org/10.1007/s00122-013-2111-zen_US
cg.isijournalISI Journalen_US
cg.issn0040-5752en_US
cg.issue8en_US
cg.journalTAG Theoretical and Applied Geneticsen_US
cg.subject.agrovocsynaptonemal complexen_US
cg.subject.agrovocWheaten_US
cg.volume126en_US
dc.contributorKumar, Ajayen_US
dc.contributorZhang, Qifengen_US
dc.contributorPaux, Etienneen_US
dc.contributorHuttner, Ericen_US
dc.contributorKilian, Andrzejen_US
dc.contributorDizon, Rhoderissaen_US
dc.contributorFeuillet, Catherineen_US
dc.contributorXu, S. S.en_US
dc.contributorKianian, Shahryar F.en_US
dc.creatorBassi, Filippoen_US
dc.date.accessioned2017-07-23T23:21:16Z
dc.date.available2017-07-23T23:21:16Z
dc.description.abstractSince the dawn of wheat cytogenetics, chromosome 3B has been known to harbor a gene(s) that, when removed, causes chromosome desynapsis and gametic sterility. The lack of natural genetic diversity for this gene(s) has prevented any attempt to fine map and further characterize it. Here, gamma radiation treatment was used to create artificial diversity for this locus. A total of 696 radiation hybrid lines were genotyped with a custom mini array of 140 DArT markers, selected to evenly span the whole 3B chromosome. The resulting map spanned 2,852 centi Ray with a calculated resolution of 0.384 Mb. Phenotyping for the occurrence of meiotic desynapsis was conducted by measuring the level of gametic sterility as seeds produced per spikelet and pollen viability at booting. Composite interval mapping revealed a single QTL with LOD of 16.2 and r2 of 25.6 % between markers wmc326 and wPt-8983 on the long arm of chromosome 3B. By independent analysis, the location of the QTL was confirmed to be within the deletion bin 3BL7-0.63-1.00 and to correspond to a single gene located ~1.4 Mb away from wPt-8983. The meiotic behavior of lines lacking this gene was characterized cytogenetically to reveal striking similarities with mutants for the dy locus, located on the syntenic chromosome 3 of maize. This represents the first example to date of employing radiation hybrids for QTL analysis. The success achieved by this approach provides an ideal starting point for the final cloning of this interesting gene involved in meiosis of cereals.en_US
dc.formatPDFen_US
dc.identifierhttps://link.springer.com/article/10.1007%2Fs00122-013-2111-zen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/xFhJ2e9K/v/c94e34e729083678af6e9c2d66b89464en_US
dc.identifier.citationFilippo Bassi, Ajay Kumar, Qifeng Zhang, Etienne Paux, Eric Huttner, Andrzej Kilian, Rhoderissa Dizon, Catherine Feuillet, S. S. Xu, Shahryar F. Kianian. (31/8/2013). Radiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheat. TAG Theoretical and Applied Genetics, 126 (8), pp. 1977-1990.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/7229
dc.languageenen_US
dc.publisherSpringer Verlag (Germany)en_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceTAG Theoretical and Applied Genetics;126,(2013) Pagination 1977-1990en_US
dc.subjectpollen viabilityen_US
dc.subjectchinese springen_US
dc.subjectdart markeren_US
dc.subjectpollen mother cellen_US
dc.titleRadiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheaten_US
dc.typeJournal Articleen_US
dcterms.available2013-05-23en_US
dcterms.extent1977-1990en_US
dcterms.issued2013-08-31en_US
mel.impact-factor4.132en_US

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