Aloe vera L. (Asphodelaceae): Supplementation of in-vitro culture medium with Aloe vera gel for production of genetically stable plants

cg.contactabdennacer.boulila@inrap.rnrt.tnen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerEcole Supérieure d'Agriculture de Mateur - ESAMen_US
cg.contributor.centerUniversity of Carthage - UCAR Tunisen_US
cg.contributor.centerNational Institute of Research and Physico-chemical Analyses, Biotechpole of Sidi Thabeten_US
cg.contributor.centerNational Institute of Applied Science and Technologyen_US
cg.contributor.crpCGIAR Research Program on Livestock Agri-Food Systems - Livestocken_US
cg.contributor.crpResilient Agrifood Systems - RAFSen_US
cg.contributor.funderInternational Livestock Research Institute - ILRIen_US
cg.contributor.projectCGIAR Research Program on Livestock Agri-Food Systemsen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryTNen_US
cg.coverage.regionNorthern Africaen_US
cg.creator.idSlim, Slim: 0000-0001-8294-0685en_US
cg.creator.idLouhaichi, Mounir: 0000-0002-4543-7631en_US
cg.date.embargo-end-dateTimelessen_US
cg.identifier.doihttps://dx.doi.org/10.1016/j.sajb.2021.08.025en_US
cg.isijournalISI Journalen_US
cg.issn0254-6299en_US
cg.journalSouth African Journal of Botanyen_US
cg.subject.actionAreaResilient Agrifood Systemsen_US
cg.subject.agrovocmolecular markersen_US
cg.subject.agrovocgenetic stabilityen_US
dc.contributorYangui, Islemen_US
dc.contributorSanaa, Adnenen_US
dc.contributorSlim, Slimen_US
dc.contributorLouhaichi, Mouniren_US
dc.contributorMessaoud, Chokrien_US
dc.contributorBoulila, Abdennaceren_US
dc.contributorBettaieba, Taoufiken_US
dc.creatorHamdeni, Imtineneen_US
dc.date.accessioned2021-09-28T20:50:03Z
dc.date.available2021-09-28T20:50:03Z
dc.description.abstractAs in vitro culture environments can be mutagenic, it's challenging to produce true-to-true type plants from meristems. Therefore, the present study aimed to devlop an efficient protocol for micropropagation from shoot tip explants of medicinally important Aloe vera using different concentrations of A. vera leaf gel (AvG) as organic supplement to the culture media. Assessment of the genetic stability of A. vera in vitro multiplied plants compared to the donor plant is one of the most important pre-requisites especially before its use for commercial and medicinal purposes. For shoot multiplication, the highest number of axillary shoots recorded was 13.27 ±5.11 with an average length of 2.21 ±0.84 cm on M3 medium (MS + 0.2 mg L−1 of β-indole butyric acid (IBA) + 3 mg L−1 benzylamino-purine (BA) + 5% AvG). All three strength of MS medium (1, 1/2, 1/4) with different concentrations of AvG resulted in 100% root induction after 4 weeks of culture. All the rooted microshoots (100%) were successfully acclimatized. To provide a reliable conclusion on its nutritive supply to MS medium, the total phenolic (TPC), condensed tannins (TTC), flavonoid (TFC), and flavonol (TFlC) content as well as the antioxydant activity of AvG were investigated. TPC and TTC values were found to be 31.02 ± 0.46 μg gallic acid equivalent mg−1 of extract and 4.00 ± 0.96 μg catechin equivalent mg−1 of extract, respectively while TFC and TFlC were 13.58 ± 0.84 and 9.75 ± 0.58 μg rutin equivalent mg−1 of extract, respectively. The results of antioxydant activity of AvG following DPPH, FRAP and ABTS assays were equal to 12.50 ± 0.89; 49.59 ± 2.07 and 64.93 ± 3.23 µmol Trolox equivalent mg−1 of extract, respectively which may explain the positive role of AvG as supplement. The genetic stability of in vitro propagated A. vera plants was evaluated using 10 inter simple sequence repeat (ISSR), 10 start codon targeted (SCoT) and 10 simple sequence repeat (SSR) markers. Out of these, 5 ISSR, 3 SCoT and 6 SSR primers produced resolvable, reproducible and scorable bands. ISSR primers generated 32 amplification products ranging from 200 to 1040 bp in size with a mean number of 6.4 bands while SCoT primers generated 33 amplicons with an average of 11 bands in the range of 160–2410 bp. In the SSR products, 6 bands were observed, each primer produced one band ranging from 410 to 720 bp. All banding profiles were monomorphic proving that the stability of the micropropagated A. vera plants was maintained. This study proved the suitability of supplementing in vitro culture media with AvG to enhance direct regeneration through shoot tip explants of A. vera for large scale micropropagation applications.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifier.citationImtinene Hamdeni, Islem Yangui, Adnen Sanaa, Slim Slim, Mounir Louhaichi, Chokri Messaoud, Abdennacer Boulila, Taoufik Bettaieba. (9/9/2021). Aloe vera L. (Asphodelaceae): Supplementation of in-vitro culture medium with Aloe vera gel for production of genetically stable plants. South African Journal of Botany.en_US
dc.identifier.statusTimeless limited accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/66160
dc.languageenen_US
dc.publisherElsevier (12 months)en_US
dc.sourceSouth African Journal of Botany;(2021)en_US
dc.subjectin vitro propagationen_US
dc.subjectaloe vera leaf gelen_US
dc.subjectbiochemical activitiesen_US
dc.subjectantioxydant assaysen_US
dc.titleAloe vera L. (Asphodelaceae): Supplementation of in-vitro culture medium with Aloe vera gel for production of genetically stable plantsen_US
dc.typeJournal Articleen_US
dcterms.available2021-09-09en_US
mel.impact-factor2.315en_US
mel.project.openhttps://mel.cgiar.org/projects/237en_US

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