Genetic dissection of seed-iron and zinc concentrations in chickpea
cg.contact | H.UPADHYAYA@CGIAR.ORG | en_US |
cg.contributor.center | International Crops Research Institute for the Semi-Arid Tropics - ICRISAT | en_US |
cg.contributor.center | National Institute of Plant Genome Research - NIPGR | en_US |
cg.contributor.center | Indian Council of Agricultural Research, National Research Centre on Plant Biotechnology - ICAR- NRCPB | en_US |
cg.contributor.crp | CGIAR Research Program on Grain Legumes - GL | en_US |
cg.contributor.funder | CGIAR System Organization - CGIAR | en_US |
cg.contributor.project-lead-institute | International Crops Research Institute for the Semi-Arid Tropics - ICRISAT | en_US |
cg.coverage.country | IN | en_US |
cg.coverage.region | Southern Asia | en_US |
cg.identifier.doi | https://dx.doi.org/10.1038/srep24050 | en_US |
cg.isijournal | ISI Journal | en_US |
cg.issn | 2045-2322 | en_US |
cg.journal | Scientific Reports | en_US |
cg.subject.agrovoc | qtl mapping | en_US |
cg.subject.agrovoc | genetic markers | en_US |
cg.subject.agrovoc | genetics | en_US |
cg.subject.agrovoc | chickpeas | en_US |
cg.subject.agrovoc | chickpea | en_US |
cg.volume | 6:24050 | en_US |
dc.contributor | Bajaj, Deepak | en_US |
dc.contributor | Das, Shouvik | en_US |
dc.contributor | kumar, vinod | en_US |
dc.contributor | Gowda, C. L. L. | en_US |
dc.contributor | Sharma, Shivali | en_US |
dc.contributor | Tyagi, Akhilesh K. | en_US |
dc.contributor | Parida, Swarup K. | en_US |
dc.creator | Upadhyaya, Hari D. | en_US |
dc.date.accessioned | 2017-04-20T10:42:48Z | |
dc.date.available | 2017-04-20T10:42:48Z | |
dc.description.abstract | The SNP-based high-resolution QTL mapping mapped eight major genomic regions harbouring robust QTLs governing seed-Fe and Zn concentrations (39.4% combined phenotypic variation explained/PVE) on six chromosomes of an intra-specific high-density genetic linkage map (1.56 cM map-density). 24620 SNPs discovered from genome-wide GBS (genotyping-by-sequencing) and 13 known cloned Fe and Zn contents-related chickpea gene-orthologs were genotyped in a structured population of 92 sequenced desi and kabuli accessions. The large-scale 16591 SNP genotyping- and phenotyping-based GWAS (genome-wide association study) identified 16 genomic loci/genes associated (29% combined PVE) with seed-Fe and Zn concentrations. Of these, 11 trait-associated SNPs in the genes linked tightly with eight QTLs were validated by QTL mapping. The seed-specific expression, including pronounced differential-regulation of 16 trait-associated genes particularly in accessions/mapping individuals with contrasting level of seed-Fe and Zn contents was apparent. Collectively, the aforementioned rapid integrated genomic strategy led to delineate novel functional non-synonymous and regulatory SNP allelic-variants from 16 known/candidate genes, including three strong trait-associated genes (encoding late embryogenesis abundant and yellow stripe-like 1 protein, and vacuolar protein sorting-associated protein) and eight major QTLs regulating seed-Fe and Zn concentrations in chickpea. These essential inputs thus have potential to be deployed in marker-assisted genetic enhancement for developing nutritionally-rich iron/zinc-biofortified chickpea cultivars. | en_US |
dc.format | en_US | |
dc.identifier | https://mel.cgiar.org/reporting/downloadmelspace/hash/pS3mhRin/v/35a085e572759c25d9ea75aaa1fc2ace | en_US |
dc.identifier.citation | Hari D. Upadhyaya, Deepak Bajaj, Shouvik Das, vinod kumar, C. L. L. Gowda, Shivali Sharma, Akhilesh K. Tyagi, Swarup K. Parida. (11/4/2016). Genetic dissection of seed-iron and zinc concentrations in chickpea. Scientific Reports, 6: 24050. | en_US |
dc.identifier.status | Open access | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/6842 | |
dc.language | en | en_US |
dc.publisher | Nature Research (part of Springer Nature) (Fully open access journals) | en_US |
dc.rights | CC-BY-4.0 | en_US |
dc.source | Scientific Reports; 6:24050,(2016) | en_US |
dc.subject | genetic | en_US |
dc.subject | genome widea ssociation studies | en_US |
dc.title | Genetic dissection of seed-iron and zinc concentrations in chickpea | en_US |
dc.type | Journal Article | en_US |
dcterms.available | 2016-04-11 | en_US |
mel.impact-factor | 4.122 | en_US |