Fermentation of Pretreated High-Biomass Sorghum Hydrolysates to Biohydrogen by Mixed Consortia
Views
0% 0
Downloads
0 0%
Limited access
Loading...
View/Open
Date
2016-06-30
Date Issued
ISI Journal
Impact factor: 0.621 (Year: 2016)
Authors
Citation
Suhas Wani. (30/6/2016). Fermentation of Pretreated High-Biomass Sorghum Hydrolysates to Biohydrogen by Mixed Consortia. Sugar Tech, 18 (3), pp. 266-272.
Abstract
In the present study, hydrolysate generated
during pretreatment of high-biomass sorghum as a carbon
source for biohydrogen production was investigated. The
high-biomass sorghum bagasse (HBS) was pretreated using
acid (H2SO4) and alkali (NaOH) at various concentrations
(0.5–5 % w/v) for the residence time of 30 min at 121 C,
15 lbs pressure at 10 % (w/v) solid loading. At the optimal
acid load of 2 % (w/v) H2SO4 yielded 78.0 g COD/L of
hydrolysate. The hydrolysate generated during this pretreatment
was analyzed and noticed to contain glucose
10 g L-1, xylose 23 g L-1, arabinose 2.0 g L-1, HMF 1.9
g L-1, furfural 3.5 g-1, acetic acid 9.3 g L-1, formic acid
5.0 g L-1, and phenols 1.9 g L-1. The fermentation studies
were conducted in dark conditions using all the hydrolysates
by heat-treated mixed microbial consortia. Maximum
H2 production rate (HPR), cumulative H2 production
(CHP), and specific H2 yield (SHY) were measured.
Maximum CHP (328 mL) and SHY (4.68 mol/kg CODr)
were registered with acid treatment-resulted hydrolysate,
and volatile fatty acid analysis indicated higher acetic acid
concentration (1.6 g L-1) showing acidogenic microenvironment
directing fermentation toward acetate pathway.
The present study assumes importance in safe disposal and
simultaneous production of value-added byproducts during
lignocellulosic biorefinery.
