Characterization of grain iron and zinc in lentil (Lens culinaris Medikus culinaris) and analysis of their genetic diversity using SSR markers

cg.contactharishmkhija.gene@gmail.comen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerIndian Council of Agricultural Research, Indian Agricultural Research Institute - ICAR-IARIen_US
cg.contributor.crpCGIAR Research Program on Agriculture for Nutrition and Health - A4NHen_US
cg.contributor.funderHarvestPlusen_US
cg.contributor.projectDevelopment of Lentil Cultivar with High Concentration of Iron and Zinc (HarvestPlus Program)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.creator.idSarker, Ashutosh: 0000-0002-9074-4876en_US
cg.issn1835-2693en_US
cg.issue7en_US
cg.journalAustralian journal of crop scienceen_US
cg.subject.agrovocanalysisen_US
cg.volume8en_US
dc.contributorSarker, Ashutoshen_US
dc.creatorKumar, Harishen_US
dc.date.accessioned2017-01-22T18:20:24Z
dc.date.available2017-01-22T18:20:24Z
dc.description.abstractForty-one elite lentil lines were studied for stability of grain Fe and Zn concentration across three locations (New Delhi, Ludhiana and Pantnagar) in India. The stability analysis was carried using Eberhart and Russel’s stability model. Pooled analysis of variance over locations revealed highly significant differences between genotypes, locations and genotype × location interaction. The maximum mean for grain Fe concentration over the locations was obtained for L 4704 (136.91 mg/kg grain), while for grain Zn concentration was highest for VL 141 (81.542 mg/kg grain). The highest mean grain Fe and Zn was recorded at Indian Agricultural Research Institute, New Delhi (Fe-87.30 mg/kg and Zn-68.602 mg/kg). Although both micronutrients were influenced by environment, grain Fe showed more G × E interaction in comparison to grain Zn concentration. Six lentil genotypes exhibited stability for grain Fe concentration and fifteen genotypes for grain Zn concentration were identified. The studied genotypes with diverse micronutrients concentration were analysed for molecular diversity using 32 polymorphic SSR markers. These markers amplified a total of 130 bands with PIC value ranging from 0.138 to 0.798. Based on SSR allelic diversity, genotypes were grouped in two major clusters. The clustering pattern indicated variability in the studied lines. On the basis of genetic diversity for micronutrient concentration, ten crosses are suggested for hybridization purpose to obtain the transgressive segregants. The combined analysis of multi-location phenotyping and genetic diversity further suggests six potential crosses for developing micronutrient rich varieties for the future.en_US
dc.formatPDFen_US
dc.identifierhttp://www.cropj.com/kumar_8_7_2014_1005_1012.pdfen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/3FLJH3aD/v/0277197c475b2ba457c4edc6d0ae6484en_US
dc.identifier.citationHarish Kumar, Ashutosh Sarker. (31/7/2014). Characterization of grain iron and zinc in lentil (Lens culinaris Medikus culinaris) and analysis of their genetic diversity using SSR markers. Australian journal of crop science, 8 (7), pp. 1005-1012.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/5536
dc.languageenen_US
dc.publisherSouthern Cross Publishing Groupen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceAustralian journal of crop science;8,(2014) Pagination 1005-1012en_US
dc.subjectgeneticen_US
dc.titleCharacterization of grain iron and zinc in lentil (Lens culinaris Medikus culinaris) and analysis of their genetic diversity using SSR markersen_US
dc.typeJournal Articleen_US
dcterms.available2014-07-31en_US
dcterms.extent1005-1012en_US
mel.project.openhttp://www.harvestplus.org/en_US

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