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dc.contributorSun, Weien_US
dc.contributorZou, Shungxiaen_US
dc.contributorChen, Lingen_US
dc.contributorMwacharo, Joramen_US
dc.contributorWang, Jinyuen_US
dc.creatorLv, Xiaoyangen_US
dc.date2019-11-01en_US
dc.date.accessioned2020-02-15T16:54:54Z
dc.date.available2020-02-15T16:54:54Z
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/69e89ffacec2f7f3c80789c8e08ccd2ben_US
dc.identifier.citationXiaoyang Lv, Wei Sun, Shungxia Zou, Ling Chen, Joram Mwacharo, Jinyu Wang. (1/11/2019). Characteristics of the BMP7 Promoter in Hu Sheep. Animals, 9 (11).en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/10730
dc.description.abstractThe BMP7 gene is involved in the growth and development of hair follicles but its regulation mechanism is unclear. We studied the regulation mechanism of the BMP7 promoter by cloning the proximal promoter of BMP7 for bioinformatics analysis. A series of missing vectors was then constructed for dual-fluorescein activity detection based on the bioinformatics analysis results. Wetestedtranscription-factorbinding-sitemutationsandtranscriptionfactorover-expression to analyze the transcriptional regulation principle of the BMP7 promoter region. The upstream transcriptionalregulatoryregionoftheBMP7geneproximalpromoterwaspredictedbybioinformatics. Therewere−1216bpto−1166bpand−632bpto−582bptranscriptioninitiationsitesintheupstream transcriptionalregulatoryregionoftheBMP7geneproximalpromoter. TheCpGislands’distribution showed that there were many CpG islands at−549 bp to 1 bp. A dual-luciferase assay revealed high activity between−758 bp and−545 bp in the core region and a possible binding site for transcription factors SP1 and EGR1. The transcriptional activity of BMP7 was significantly decreased in the transcriptional regulatory region of the BMP7 after EGR1 and SP1 mutation. Transcription was significantly enhanced by over expression of the EGR1 transcription factor, which strongly suggests that EGR1 and SP1 play important roles in BMP7 regulation.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherMDPIen_US
dc.rightsCC-BY-4.0en_US
dc.sourceAnimals;9,(2019)en_US
dc.subjecttranscription factoren_US
dc.subjecthu sheepen_US
dc.subjectbmp7en_US
dc.subjectpromoteren_US
dc.subjectSheepen_US
dc.titleCharacteristics of the BMP7 Promoter in Hu Sheepen_US
dc.typeJournal Articleen_US
cg.creator.idMwacharo, Joram: 0000-0001-6981-8140en_US
cg.creator.ID-typeORCIDen_US
cg.contributor.centerYangzhou University, College of Animal Science and Technology - YZU - CASTen_US
cg.contributor.centerYangzhou University, MOE Joint International Research Laboratory of Agriculture and Agri-Product Safety - YZU - MOE JIRLAAPSen_US
cg.contributor.centerBureau of Suzhou City, Animal Science and Veterinary Medicineen_US
cg.contributor.centerSuzhou Stud Farmen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.crpCGIAR Research Program on Livestock Agri-Food Systems - LAFSen_US
cg.contributor.funderInternational Livestock Research Institute - ILRIen_US
cg.contributor.projectCGIAR Research Program on Livestock Agri-Food Systemsen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionEastern Asiaen_US
cg.coverage.countryCNen_US
cg.contactdkxmsunwei@163.comen_US
cg.identifier.doihttps://dx.doi.org/10.3390/ani9110874en_US
dc.identifier.statusOpen accessen_US
mel.project.openhttps://mel.cgiar.org/projects/237en_US
mel.impact-factor1.832en_US


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