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Assessment of genetic diversity for cotton leaf curl virus (CLCuD), fiber quality and some morphological traits using different statistical procedures in Gossypium hirsutum L
Cotton leaf curl virus disease (CLCuD) is one of the major factors limiting cotton production in Pakistan. For the assessment of genetic diversity one hundred cotton genotypes was evaluated for CLCuD tolerance, fiber ...
Xanthium strumarium: a weed host of components of begomovirus-betasatellite complexes affecting crops
Xanthium strumarium is a common weed that often shows symptoms typical of begomovirus infection, such as leaf curling and vein thickening. The virus complex isolated from the weed consisted of two begomoviruses along with ...
Nutrients Can Enhance the Abundance and Expression of Alkane Hydroxylase CYP153 Gene in the Rhizosphere of Ryegrass Planted in Hydrocarbon-Polluted Soil
Plant-bacteria partnership is a promising strategy for the remediation of soil and water polluted with hydrocarbons. However, the limitation of major nutrients (N, P and K) in soil affects the survival and metabolic activity ...
Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus
Background: Cotton leaf curl disease, caused by single-stranded DNA viruses of the genus Begomovirus (family Geminiviridae), is a major constraint to cotton cultivation across Pakistan and north-western India. At this time ...
Amplicon-based RNA interference targeting V2 gene of cotton leaf curl kokhran virus- burewala strain can provide resistance in transgenic cotton plants
The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation ...
First Report of Chilli leaf curl virus and Associated Alpha- and Beta-satellite DNAs Infecting Nettle Weed (Urtica dioica) in Pakistan
Weeds act as reservoirs of begomoviruses and ‘‘mixing vessels’’ leading to emergence of new viruses that may infect crops once a susceptible host plant is introduced into an agro-ecological system. Owing to the importance ...
Virus-Induced Gene Silencing in Cultivated Cotton (Gossypium spp.) Using Tobacco Rattle Virus.
The study described here has optimized the conditions for virus-induced gene silencing (VIGS) in three cultivated cotton species (Gossypium hirsutum, G. arboreum, and G. herbaceum) using a Tobacco rattle virus (TRV) vector. ...