Effects of exogenous brassinosteroids on the starch structure, physicochemical properties and digestibility of wheat under high-temperature stress at the early grain-filling stage

Abstract

The starch synthesis of wheat was seriously affected by high temperature stress, which affected its yield and quality. At the same time, plant hormones are often used to regulate crop production and mitigate environmental stress in times of adversity. However, whether Brassinosteroids (BRs) can alleviate the adverse effects of high-temperature stress on wheat starch and its internal mechanism are still unclear. Therefore, a two-year field experiment was carried out from 2020 to 2022 to explore the effects of exogenous BRs spraying on the wheat yield, morphological structure, and physicochemical properties of starch under high-temperature stress at the early grain-filling stage. The results showed that exogenous spraying of BRs under high-temperature stress increased the activity of key enzymes involved in starch synthesis, the contents of amylose (25.34 %) and amylopectin (11.57 %) were increased on average in two years and ultimately increased the yield. On the other hand, BRs increased the number of B-type (49.90 %) and C-type starch granules (23.19 %), decreased the number of A-type starch granules (6.38 %) under high-temperature stress, and reduced the chain length of B1 (5.04 %) and B2 (4.88 %) chains of amylopectin. Moreover, BRs decreased crystallinity (25.40 %), gelatinization enthalpy (4.76 %) and 1047/1022 cm (1.25 %), thereby improving the cooking quality of the starch. Through further analysis of the rheological properties and digestibility of starch, BRs improved the processing quality of starch and increased the content of resistant starch (RS, 15.77 %), which provided insights for the processing and utilization of food. In summary, under high-temperature stress, exogenous BRs increased wheat yield and improved starch quality. We recommend BRs as an effective management measure to mitigate high-temperature stress to cope with future climate change.