Arbuscular Mycorrhizal Fungal (AMF) Inoculum Production Training - Report
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Sigrun Dahlin, Joyce Mnyazi Jefwa. (17/11/2018). Arbuscular Mycorrhizal Fungal (AMF) Inoculum Production Training - Report.
Abstract
Summary
The Arbuscular Mycorrhizal (AM) fungal inoculum production training was
conducted from the 12th November – 17th November 2018. The aim of the
training was to build capacity in methods of inoculum production.
According to the background of the participants, the training programme
activities were tailored to meet this objective. The training therefore focused
on methods of AMF propagule diagnosis and AMF colonization, which form
the basis of inoculum production and evaluation for effectiveness. This was
then followed by methods of inoculum production, assessment of inoculum
vigor, and exposure to methods of application and storage.
Throughout the training, biosaftey was stressed with the following measures
emphasized: laboratory handling of chemicals (dyes) and equipment
(Microscope, centrifuge and autoclave).
The mode of training was through lectures, practical’s and demonstration
sessions. The activities undertaken are classified into six major sessions:
(1) Introductory session on mycorrhzal symbiosis (2) characteristics of
Arbuscular Mycorrhizal Fungi (3) AMF Inoculants (4) practical session on
Root clearing, staining and observation (5) practical training on Spore
extraction, distinguishing spores under stereo-microscope, mounting and
observation under compound microscope (6) AMF Culture initiation and
inoculum production: trap cultures, spore and root cultures.
