Expression and localization of PIWI proteins in testis and ovary of domestic sheep

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cg.contactmxchu@263.neten_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerChina Agricultural University - CAU Chinaen_US
cg.contributor.centerChinese Academy of Agricultural Sciences, Institute of Animal Science - CAAS - IASen_US
cg.contributor.centerAnhui Agricultural University - ANHUIen_US
cg.contributor.crpCGIAR Research Program on Livestock Agri-Food Systems - Livestocken_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.creator.idMwacharo, Joram: 0000-0001-6981-8140en_US
cg.identifier.doihttps://dx.doi.org/10.17221/7/2020-CJASen_US
cg.isijournalISI Journalen_US
cg.issn1212-1819en_US
cg.issue3en_US
cg.journalCzech Journal of Animal Scienceen_US
cg.subject.agrovocreproductionen_US
cg.volume65en_US
dc.contributorLiu, Qiuyueen_US
dc.contributorWang, Xiangyuen_US
dc.contributorHu, Wenpingen_US
dc.contributorHan, Depingen_US
dc.contributorMwacharo, Joramen_US
dc.contributorWei, Caihongen_US
dc.contributorChu, Mingxingen_US
dc.contributorDi, Ranen_US
dc.creatorLi, Chunyanen_US
dc.date.accessioned2020-10-07T07:37:16Z
dc.date.available2020-10-07T07:37:16Z
dc.description.abstractThe piRNA-PIWI protein complex plays crucial roles in safeguarding the genome against inordinate transposon mobilization and regulation of embryonic development. A previous study indicated the presence of piRNA in sheep reproductive organs. However, the tissue distribution and cellular localization of PIWI proteins in sheep remains unclear. Therefore the present study aimed to explore the expression profiles of mRNAs of mammalian PIWI proteins (PIWIL1, PIWIL2, PIWIL4 and AGO3) in 9 tissues derived from adult male and female sheep. Results showed the expression of PIWIL1, PIWIL2, and PIWIL4 was significantly higher in the testis and ovary than in the other tissues. Immunohistochemistry analysis of testes indicated that each of the 4 proteins had specific cellular localizations, and some of the localizations were different from those of other species. All the proteins were mainly localized in the primary spermatocytes, suggesting that they are crucial for silencing of transposon to guarantee the integrity of the gamete genome during early stage of spermatogenesis. In the ovaries, the PIWI proteins were mainly localized in oocytes from antral follicles and leukocytes in ovarian blood. Our results provide insights to better understand the functions of PIWI proteins during spermatogenesis, oogenesis and immune defence in sheep.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/82b93c7ea96cb8d3726582b51b3c9345/v/5a6817f5141124d6f47b0d1b0d4c3c9ben_US
dc.identifier.citationChunyan Li, Qiuyue Liu, Xiangyu Wang, Wenping Hu, Deping Han, Joram Mwacharo, Caihong Wei, Mingxing Chu, Ran Di. (3/3/2020). Expression and localization of PIWI proteins in testis and ovary of domestic sheep. Czech Journal of Animal Science, 65 (3), pp. 86-96.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/11859
dc.languageenen_US
dc.publisherCzech Academy of Agricultural Sciencesen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceCzech Journal of Animal Science;65,(2020) Pagination 86-96en_US
dc.subjectlocalizationen_US
dc.subjectpiwi proteinsen_US
dc.subjecttestisen_US
dc.subjectovaryen_US
dc.titleExpression and localization of PIWI proteins in testis and ovary of domestic sheepen_US
dc.typeJournal Articleen_US
dcterms.available2020-03-03en_US
dcterms.extent86-96en_US
mel.impact-factor0.835en_US

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