In vitro assessment of the anthelmintic activity of Hedysarum carnosum Desf. at different phenological stages and from six locations in Tunisia

cg.contactasaissa@laposte.neten_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerInstitut Supèrieur Agronomique de Chott Mariem - ISA-CMen_US
cg.contributor.centerEcole Nationale Vétérinaire de Toulouse - INRA-ENVTen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryTNen_US
cg.coverage.regionNorthern Africaen_US
cg.date.embargo-end-dateTimelessen_US
cg.identifier.doihttps://dx.doi.org/10.1017/S0031182016000317en_US
cg.isijournalISI Journalen_US
cg.issn0031-1820en_US
cg.issue6en_US
cg.journalParasitologyen_US
cg.subject.agrovocnematodesen_US
cg.subject.agrovoclocationen_US
cg.subject.agrovoctanninsen_US
cg.volume143en_US
dc.contributorManolaraki, Foteinien_US
dc.contributorBen Salem, Hichemen_US
dc.contributorHoste, Hervéen_US
dc.contributorKraiem, Khemaisen_US
dc.creatorAissa, Asmaen_US
dc.date.accessioned2022-02-15T23:12:04Z
dc.date.available2022-02-15T23:12:04Z
dc.description.abstractGastrointestinal nematodes are compromising productivity of grazing sheep and goats. Therefore, scientists have been looking for cost-effective alternative options. Forage legumes (Fabacea Family) contain tannins that could improve livestock performance and their health as well. The present study aimed to (i) determine the in vitro anthelmintic (AH) activity of 19 acetonic extracts of Hedysarum carnosum Desf on Haemonchus contortus by a larval exsheathment assay (LEA); (ii) test the anthelmintic activity of condensed tannins using a deactivating reagent, polyvinylpolypyrrolidone (PVPP); (iii) study the effect of location and the phenological stage on the percentage of exsheathment. The LEA was used at different concentrations (150, 300, 600, 1200 mu g mL(-1) of acetonic extract/mL of purified buffer solution (PBS)). The larval exsheathment is concentration, location, phenological stage dependent. All extracts, caused a delay of the percentage of exsheathment over 50% so the AH activity of H. carnosum was confirmed. After addition of PVPP, the % exsheathment was similar to the 150 mu g mL(-1) concentration. The biplot showed that Loc1(S), Loc4(B), Loc 5(PF), Loc 6(BM) and Loc 6(PF) were isolated from other plant extract sample. Our in vitro study showed that H. carnosum seems to be a promising alternative to AH drugs.en_US
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifier.citationAsma Aissa, Foteini Manolaraki, Hichem Ben Salem, Hervé Hoste, Khemais Kraiem. (6/5/2016). In vitro assessment of the anthelmintic activity of Hedysarum carnosum Desf. at different phenological stages and from six locations in Tunisia. Parasitology, 143 (6), pp. 778-786.en_US
dc.identifier.statusTimeless limited accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/67056
dc.languageenen_US
dc.publisherCambridge University Press (CUP)en_US
dc.sourceParasitology;143,(2016) Pagination 778-786en_US
dc.subjecthedysarum carnosum desfen_US
dc.subjectlarval exsheathment assayen_US
dc.titleIn vitro assessment of the anthelmintic activity of Hedysarum carnosum Desf. at different phenological stages and from six locations in Tunisiaen_US
dc.typeJournal Articleen_US
dcterms.available2016-05-06en_US
dcterms.extent778-786en_US
mel.impact-factor3.234en_US

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