Selection of Tolerant Lines to Salinity Derived from Durum Wheat (Triticum durum Desf.) in Vitro Culture

cg.contactolfa.slama@planet.tnen_US
cg.contributor.centerNational Agronomic Institute of Tunisia - INATen_US
cg.contributor.centerEcole Supérieure d’Agriculture du Kef - ESA Kefen_US
cg.contributor.crpCGIAR Research Program on Dryland Systems - DSen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderInternational Fund for Agricultural Development - IFADen_US
cg.contributor.projectEnhanced small-holder wheat-legume cropping systems to improve food security under changing climate in the drylands of West Asia and North Africaen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryTNen_US
cg.coverage.regionNorthern Africaen_US
cg.issn0976-6898en_US
cg.journalAgricultural Sciencesen_US
cg.subject.agrovocgynogenesisen_US
cg.subject.agrovocsalinity toleranceen_US
cg.volume6en_US
dc.contributorAyed, Sourouren_US
dc.contributorSlim-Amara, Hajeren_US
dc.creatorAyed-Slama, O.en_US
dc.date.accessioned2017-11-28T14:36:49Z
dc.date.available2017-11-28T14:36:49Z
dc.description.abstractThe genetic variability is considered as the major principle of plant breeding for durum wheat. This variability can be induced in vitro by selection pressure exerted by stress factors such as salinity in order to regenerate the vitro plantlets tolerant. This study aims in the first step in the regeneration of plantlets tolerant to salinity from mature embryos culture derived from two Tunisian durum wheat varieties: improved (Razzek) and landrace (Jenah Khotifa (JK)) varieties. The tolerance evaluation to salt stress was applied in vitro (100 mmol∙l−1 NaCl) and was based on various parameters. Our results showed that JK variety was distinguished by a stable response for all parameters tested: average weight of callus (368.1 mg for control and 307 mg under salt stress), callus regenerated percentage (36.6% for control and 35.7% under salt stress) and green shoots number/callus (17 for control and 17 under salt stress). This stability of response translates the adaptability of this variety to salinity. In order to fix regenerated JK plantlets in single generation and obtain HDs homozygous stable lines, in vitro gynogenesis technical is tested for this genotype. The Evaluation of gynogenetic capacity focused on about 1200 unfertilized ovaries of JK and was based on its ability to induction, differentiation, development of green shoots, and haploid plantlets regeneration. JK showed good tolerance to salinity and a relatively good response to gynogenesis.en_US
dc.formatPDFen_US
dc.identifierhttp://www.scirp.org/journal/PaperInformation.aspx?paperID=58178en_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/2xuuIAnV/v/366518603072c000cc7bb567873a5faben_US
dc.identifier.citationO. Ayed-Slama, Sourour Ayed, Hajer Slim-Amara. (22/7/2015). Selection of Tolerant Lines to Salinity Derived from Durum Wheat (Triticum durum Desf. ) in Vitro Culture. Agricultural Sciences, 6.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/7532
dc.languageenen_US
dc.publisherKamla-Raj Enterprisesen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceAgricultural Sciences;6,(2015)en_US
dc.subjectmature embryosys cultureen_US
dc.subjectdiversityen_US
dc.titleSelection of Tolerant Lines to Salinity Derived from Durum Wheat (Triticum durum Desf.) in Vitro Cultureen_US
dc.typeJournal Articleen_US
dcterms.available2015-07-22en_US
mel.project.openhttps://mel.cgiar.org/projects/46en_US

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