Study of Secondary Endosymbionts of Whiteflies Collected from Cotton Growing Districts of Punjab, Pakistan
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Citation
Muhammad Ali, Imran Amin, Sohail Akhtar, Shahid Mansoor. (1/8/2016). Study of Secondary Endosymbionts of Whiteflies Collected from Cotton Growing Districts of Punjab, Pakistan. Pakistan Journal of Zoology, 48 (5), pp. 1549-1553.
Abstract
Whitefly is the insect vector for cotton leaf curl disease. Like many arthropods, whitefly possess
different primary and secondary endosymbionts depending on ecological zone and biotype. These
endosymbionts play important role in several metabolic processes including reproduction. In this
study, endosymbiont specific primers were used to check their presence in whiteflies. Each
endosymbiont primer set amplify a PCR product of specific size and thus amplification of PCR
product shows its presence in extracted DNA. Amplified DNA was checked using sodium channel
gene-specific primers as internal control and the presence of endosymbiont was confirmed using 16S
primers. We found multiple infections of endosymbionts in each district followed by confirmation
through different molecular approaches. Our results confirmed the presence of multiple/diverse
endosymbionts in whitefly samples collected from Punjab, Pakistan. The results would be helpful to
correlate the occurrence of whitefly biotypes and disease intensity in different regions of Pakistan.