Agrobacterium-mediated transformation of mature embryo tissues of bread wheat (Triticum aestivum L.) genotypes

cg.contactiraqid@yahoo.fren_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerNational Institute of Agronomic Research Morocco - INRA Moroccoen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderInternational Maize and Wheat Improvement Center - CIMMYTen_US
cg.contributor.projectCRP WHEAT Phase IIen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.creator.idUdupa, Sripada M.: 0000-0003-4225-7843en_US
cg.identifier.doihttps://dx.doi.org/10.1556/0806.45.2017.055en_US
cg.isijournalISI Journalen_US
cg.issn0133-3720en_US
cg.journalCereal Research Communicationsen_US
cg.subject.agrovocgenetic transformationen_US
cg.subject.agrovocagrobacterium tumefaciensen_US
cg.volume46en_US
dc.contributorAbdelwahd, Rabhaen_US
dc.contributorUdupa, Sripada M.en_US
dc.contributorDiria, Ghizlanen_US
dc.contributorEl-Mouhtadi, A.en_US
dc.contributorAhansal, Khadijaen_US
dc.contributorGaboun, F.en_US
dc.contributorDoulra, A.en_US
dc.contributorIraqi, Drissen_US
dc.creatorAadel, Hananeen_US
dc.date.accessioned2018-03-12T01:22:57Z
dc.date.available2018-03-12T01:22:57Z
dc.description.abstractAlthough significant progress has been made on Agrobacterium-mediated wheat transformation, current methodologies using immature embryos as recipient tissues are labor intensive, time consuming and expensive. The use of mature embryos as explants is increasingly being recognized as an optimal method for developing regenerable cell lines during wheat transformation. Therefore, we have developed an Agrobacterium-based transformation protocol using mature embryos while adjusting several factors that influence genetic transformation efficiency. In this study, we focussed on acetosyringone concentrations, genotypes and different types of mature embryos (intact or longitudinally halved-embryos or fragmented into four latitudinal pieces) used as a source of explants for the genetic transformation. A. tumefaciens strain EHA101 harboring the plasmid vector pTF101.1 carrying the barley HVA1 gene and bar-selectablemarker gene were used. Mature intact-embryos and longitudinally halved-embryos yielded the highest number of putative transgenic plantlets on the selection medium. However, no plantlets were obtained from latitudinal fragmented mature embryos. ‘Amal’ and ‘Rajae’ genotypes regenerated the highest number of putative transgenic plants and 200 μM acetosyringone was found to be the optimal concentration for their transformation. A total of 47 transgenic plants were selected with 11 plantlets showing resistance to leaf painting. Molecular analysis revealed that 1% and 0.66% of T0 regenerated plantlets were successfully transformed and carried the HVA1 gene for the ‘Amal’ and ‘Rajae’ genotypes, respectively. Additional analysis shows the transgene is stably inherited in the T1 generation. Based on the results, we conclude that among the influencing factors tested, genotypes, mature embryo explant types and acetosyringone concentration contribute significantly to the success of bread wheat transformation.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/Y4eVFtKSen_US
dc.identifier.citationHanane Aadel, Rabha Abdelwahd, Sripada M. Udupa, Ghizlan Diria, A. El-Mouhtadi, Khadija Ahansal, F. Gaboun, A. Doulra, Driss Iraqi. (2/1/2018). Agrobacterium-mediated transformation of mature embryo tissues of bread wheat (Triticum aestivum L. ) genotypes. Cereal Research Communications, 46.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/8055
dc.languageenen_US
dc.publisherAkadémiai Kiadóen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceCereal Research Communications;46,(2018)en_US
dc.subjectmature embryoen_US
dc.subjectacetosyringoneen_US
dc.titleAgrobacterium-mediated transformation of mature embryo tissues of bread wheat (Triticum aestivum L.) genotypesen_US
dc.typeJournal Articleen_US
dcterms.available2018-01-02en_US
mel.impact-factor0.489en_US

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