Genome wide association studies (GWAS) of spot blotch resistance at the seedling and the adult plant stages in a collection of spring barley
cg.contact | S.Gyawali@cgiar.org | en_US |
cg.contributor.center | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.contributor.center | Indian Council of Agricultural Research, Indian Institute of Wheat and Barley Research - ICAR-IIWBR | en_US |
cg.contributor.center | Banaras Hindu University | en_US |
cg.contributor.center | United State Department of Agriculture, Agricultural Research Service, Natural Resource Management Research - USDA-ARS Mandan | en_US |
cg.contributor.center | Narendra Deva University of Agriculture and Technology - NDUAT | en_US |
cg.contributor.crp | CRP on Dryland Cereals - DC | en_US |
cg.contributor.funder | United States Agency for International Development - USAID | en_US |
cg.contributor.project | Sustainability and Operation of the Regional Research Centers in a Number of Arab Countries (Phase II) | en_US |
cg.contributor.project-lead-institute | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.coverage.country | MA | en_US |
cg.coverage.region | Northern Africa | en_US |
cg.creator.id | Verma, Ramesh Pal Singh: 0000-0002-2621-2015 | en_US |
cg.date.embargo-end-date | Timeless | en_US |
cg.identifier.doi | https://dx.doi.org/10.1007/s11032-018-0815-0 | en_US |
cg.isijournal | ISI Journal | en_US |
cg.issn | 1380-3743 | en_US |
cg.journal | Molecular Breeding | en_US |
cg.subject.agrovoc | barley | en_US |
cg.subject.agrovoc | gwas | en_US |
cg.subject.agrovoc | bipolaris | en_US |
cg.subject.agrovoc | cochliobolus | en_US |
cg.subject.agrovoc | Barley | en_US |
cg.volume | 38:62 | en_US |
dc.contributor | Chao, Shiaoman | en_US |
dc.contributor | Vaish, Shyam | en_US |
dc.contributor | P. Singh, Shiv | en_US |
dc.contributor | Rehman, Sajid | en_US |
dc.contributor | Vishwakarma, Siya Ram | en_US |
dc.contributor | Verma, Ramesh Pal Singh | en_US |
dc.creator | Gyawali, Sanjaya | en_US |
dc.date.accessioned | 2019-01-21T20:17:48Z | |
dc.date.available | 2019-01-21T20:17:48Z | |
dc.description.abstract | Spot blotch (SB) in barley is caused by the fungal pathogen Cochliobolus sativus and considered one of the major constraints to successful barley production. Resistance to C. sativus was evaluated, using a barley collection of 336 genotypes (AM-2014), at the seedling and adult stages. Seedling resistance was evaluated by using a mixture of 19 virulent isolates in Morocco. Virulent isolates prevalent in Uttar Pradesh were used for phenotyping resistance at the adult stage in India. The AM-2014 panel was genotyped with 9-K single-nucleotide polymorphism (SNP) markers using iSelect Illumina Infinium. Genome wide association studies (GWAS) were carried out using SNP markers, infection responses, disease severity, and area under the disease progress curve (AUDPC). The mixed linear model was employed in TASSEL using principal component analysis (PCA) and Kinship matrix (K) as covariates. Higher SB severity, 82.3 ± 13.5 (mean ± SD), was recorded at the Banaras Hindu University (BHU) compared to 47.6 ± 15.0 at the Narendra Dev University of Agriculture and Technology (NDUAT). Nine QTL, Rcs-qtl-1H-126.9, Rcs-qtl-2H-148.16, Rcs-qtl-3H-25.27, Rcs-qtl-5H-80.35, Rcs-qtl-6H-58.24, Rcs-qtl-7H-29.62, Rcs-qtl-7H-29.72, Rcs-qtl-7H-32.81, and Rcs-qtl-7H-34.74, were detected for SB resistance at the seedling stage. For SB severity at the adult stage, a QTL, Rcs-qtl-7H-32.81, was detected at BHU while seven QTL, Rcs-qtl-2H-91.09, Rcs-qtl-3H-145.64, Rcs-qtl-4H-14.43, Rcs-qtl-6H-6.49, Rcs-qtl-7H-114.43, Rcs-qtl-7H-151.66, and Rcs-qtl-7H-150.36, were found for SB severity at NDUAT. Three QTL, Rcs-qtl-4H-18.61, Rcs-qtl-4H-67.91, and Rcs-qtl-5H-110.25, were significant for AUDPC of SB at BHU. The QTLs reported in this study are important to advance marker-assisted selection and gene pyramiding of SB resistance in South Asia and North Africa in future. | en_US |
dc.format | en_US | |
dc.identifier | https://mel.cgiar.org/dspace/limited | en_US |
dc.identifier | https://link.springer.com/article/10.1007/s11032-018-0815-0 | en_US |
dc.identifier.citation | Sanjaya Gyawali, Shiaoman Chao, Shyam Vaish, Shiv P. Singh, Sajid Rehman, Siya Ram Vishwakarma, Ramesh Pal Singh Verma. (3/5/2018). Genome wide association studies (GWAS) of spot blotch resistance at the seedling and the adult plant stages in a collection of spring barley. Molecular Breeding, 38: 62. | en_US |
dc.identifier.status | Timeless limited access | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/9297 | |
dc.language | en | en_US |
dc.publisher | Springer (part of Springer Nature) (Springer Open Choice Hybrid Journals) | en_US |
dc.source | Molecular Breeding;38:62,(2018) | en_US |
dc.subject | resistance | en_US |
dc.subject | audpc | en_US |
dc.subject | spotblotch | en_US |
dc.title | Genome wide association studies (GWAS) of spot blotch resistance at the seedling and the adult plant stages in a collection of spring barley | en_US |
dc.type | Journal Article | en_US |
dcterms.available | 2018-05-03 | en_US |
mel.impact-factor | 2.077 | en_US |
mel.project.open | https://mel.cgiar.org/projects/216 | en_US |