Genome wide association studies (GWAS) of spot blotch resistance at the seedling and the adult plant stages in a collection of spring barley

cg.contactS.Gyawali@cgiar.orgen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerIndian Council of Agricultural Research, Indian Institute of Wheat and Barley Research - ICAR-IIWBRen_US
cg.contributor.centerBanaras Hindu Universityen_US
cg.contributor.centerUnited State Department of Agriculture, Agricultural Research Service, Natural Resource Management Research - USDA-ARS Mandanen_US
cg.contributor.centerNarendra Deva University of Agriculture and Technology - NDUATen_US
cg.contributor.crpCRP on Dryland Cereals - DCen_US
cg.contributor.funderUnited States Agency for International Development - USAIDen_US
cg.contributor.projectSustainability and Operation of the Regional Research Centers in a Number of Arab Countries (Phase II)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryMAen_US
cg.coverage.regionNorthern Africaen_US
cg.creator.idVerma, Ramesh Pal Singh: 0000-0002-2621-2015en_US
cg.date.embargo-end-dateTimelessen_US
cg.identifier.doihttps://dx.doi.org/10.1007/s11032-018-0815-0en_US
cg.isijournalISI Journalen_US
cg.issn1380-3743en_US
cg.journalMolecular Breedingen_US
cg.subject.agrovocbarleyen_US
cg.subject.agrovocgwasen_US
cg.subject.agrovocbipolarisen_US
cg.subject.agrovoccochliobolusen_US
cg.subject.agrovocBarleyen_US
cg.volume38:62en_US
dc.contributorChao, Shiaomanen_US
dc.contributorVaish, Shyamen_US
dc.contributorP. Singh, Shiven_US
dc.contributorRehman, Sajiden_US
dc.contributorVishwakarma, Siya Ramen_US
dc.contributorVerma, Ramesh Pal Singhen_US
dc.creatorGyawali, Sanjayaen_US
dc.date.accessioned2019-01-21T20:17:48Z
dc.date.available2019-01-21T20:17:48Z
dc.description.abstractSpot blotch (SB) in barley is caused by the fungal pathogen Cochliobolus sativus and considered one of the major constraints to successful barley production. Resistance to C. sativus was evaluated, using a barley collection of 336 genotypes (AM-2014), at the seedling and adult stages. Seedling resistance was evaluated by using a mixture of 19 virulent isolates in Morocco. Virulent isolates prevalent in Uttar Pradesh were used for phenotyping resistance at the adult stage in India. The AM-2014 panel was genotyped with 9-K single-nucleotide polymorphism (SNP) markers using iSelect Illumina Infinium. Genome wide association studies (GWAS) were carried out using SNP markers, infection responses, disease severity, and area under the disease progress curve (AUDPC). The mixed linear model was employed in TASSEL using principal component analysis (PCA) and Kinship matrix (K) as covariates. Higher SB severity, 82.3 ± 13.5 (mean ± SD), was recorded at the Banaras Hindu University (BHU) compared to 47.6 ± 15.0 at the Narendra Dev University of Agriculture and Technology (NDUAT). Nine QTL, Rcs-qtl-1H-126.9, Rcs-qtl-2H-148.16, Rcs-qtl-3H-25.27, Rcs-qtl-5H-80.35, Rcs-qtl-6H-58.24, Rcs-qtl-7H-29.62, Rcs-qtl-7H-29.72, Rcs-qtl-7H-32.81, and Rcs-qtl-7H-34.74, were detected for SB resistance at the seedling stage. For SB severity at the adult stage, a QTL, Rcs-qtl-7H-32.81, was detected at BHU while seven QTL, Rcs-qtl-2H-91.09, Rcs-qtl-3H-145.64, Rcs-qtl-4H-14.43, Rcs-qtl-6H-6.49, Rcs-qtl-7H-114.43, Rcs-qtl-7H-151.66, and Rcs-qtl-7H-150.36, were found for SB severity at NDUAT. Three QTL, Rcs-qtl-4H-18.61, Rcs-qtl-4H-67.91, and Rcs-qtl-5H-110.25, were significant for AUDPC of SB at BHU. The QTLs reported in this study are important to advance marker-assisted selection and gene pyramiding of SB resistance in South Asia and North Africa in future.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifierhttps://link.springer.com/article/10.1007/s11032-018-0815-0en_US
dc.identifier.citationSanjaya Gyawali, Shiaoman Chao, Shyam Vaish, Shiv P. Singh, Sajid Rehman, Siya Ram Vishwakarma, Ramesh Pal Singh Verma. (3/5/2018). Genome wide association studies (GWAS) of spot blotch resistance at the seedling and the adult plant stages in a collection of spring barley. Molecular Breeding, 38: 62.en_US
dc.identifier.statusTimeless limited accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/9297
dc.languageenen_US
dc.publisherSpringer (part of Springer Nature) (Springer Open Choice Hybrid Journals)en_US
dc.sourceMolecular Breeding;38:62,(2018)en_US
dc.subjectresistanceen_US
dc.subjectaudpcen_US
dc.subjectspotblotchen_US
dc.titleGenome wide association studies (GWAS) of spot blotch resistance at the seedling and the adult plant stages in a collection of spring barleyen_US
dc.typeJournal Articleen_US
dcterms.available2018-05-03en_US
mel.impact-factor2.077en_US
mel.project.openhttps://mel.cgiar.org/projects/216en_US

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