Detection of Four Legume Viruses (AMV, CMV, BYMV, PSbMV) in One Multiplex RT-PCR Test Applied at ICARDA’s Seed Health and Virology Laboratory

cg.contactS.KUMARI@CGIAR.ORGen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.crpResilient Agrifood Systems - RAFSen_US
cg.contributor.funderCGIAR Trust Funden_US
cg.contributor.initiativePlant Healthen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryLBen_US
cg.coverage.regionWestern Asiaen_US
cg.creator.idKumari, Safaa: 0000-0002-4492-6257en_US
cg.creator.idMoukahel, Abdulrahman: 0000-0003-4297-771Xen_US
cg.subject.actionAreaResilient Agrifood Systemsen_US
cg.subject.agrovocplant healthen_US
cg.subject.agrovocfaba beanen_US
cg.subject.agrovoclentilen_US
cg.subject.agrovocchickpeaen_US
cg.subject.agrovocalfalfaen_US
cg.subject.agrovoclegumeen_US
cg.subject.agrovocgrass pea (lathyrus sativus)en_US
cg.subject.agrovoctrifoliumen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.impactAreaGender equality, youth and social inclusionen_US
cg.subject.impactAreaEnvironmental health and biodiversityen_US
cg.subject.sdgSDG 1 - No povertyen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.subject.sdgSDG 15 - Life on landen_US
cg.subject.sdgSDG 17 - Partnerships for the goalsen_US
dc.contributorMoukahel, Abdulrahmanen_US
dc.creatorKumari, Safaaen_US
dc.date.accessioned2024-11-29T14:26:12Z
dc.date.available2024-11-29T14:26:12Z
dc.description.abstractMany viruses have been reported affecting legume crops in West Asia and North Africa (WANA region), including Alfalfa mosaic virus (AMV, genus Alfamovirus, family Bromoviridae), Cucumber mosaic virus (CMV, genus Cucumovirus, family Bromoviridae), Bean yellow mosaic virus (BYMV) and Pea seed-borne mosaic virus (PSbMV) (genus Potyvirus, family Potyviridae). The nucleic acid of these four viruses is RNA and all are transmitted by aphids in a non-persistent manner and via seeds (including faba bean, lentil and chickpea), and pose a significant threat to the productivity of food and feed legume crops. Currently, virus detection methods rely primarily on serological tests such as ELISA and TBIA to detect viral infections. However, screening each sample for all viruses via individual virus-specific tests is both cost-prohibitive and labor-intensive. Additionally, antisera for some of these viruses are difficult to obtain. Thus, there is a clear need for a more efficient assay capable of detecting and differentiating the maximum number of these viruses in a single test, with high sensitivity to process large number of samples. The Reverse transcription polymerase chain reaction (RT-PCR) is a powerful technique for amplifying specific RNA sequences, enabling the detection of minute quantities of target molecules. Multiplex RT-PCR has been successfully applied to detect simultaneously different RNA viruses with high sensitivity. This report describes a validated molecular tool for a sensitive RT-PCR assay capable of detecting and identifying four legume viruses (AMV, CMV, BYMV, and PSbMV) in a single, straightforward test applied by ICARDA’s seed health and Virology Laboratory.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/19947f0ca2ac6723eb0f4674af4311f8en_US
dc.identifier.citationSafaa G. Kumari and Abdelrahman Moukahel. 2024. Detection of Four Legume Viruses (AMV, CMV, BYM and PSbMV) in One Multiplex RT-PCR Test Applied at ICARDA’s Seed Health and Virology Laboratory. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/69705
dc.languageenen_US
dc.publisherInternational Center for Agricultural Research in the Dry Areas (ICARDA)en_US
dc.rightsCC-BY-SA-4.0en_US
dc.subjectvirus diseaseen_US
dc.subjectseed-borneen_US
dc.subjectlegumeen_US
dc.subjectmultiplex rt-pcren_US
dc.titleDetection of Four Legume Viruses (AMV, CMV, BYMV, PSbMV) in One Multiplex RT-PCR Test Applied at ICARDA’s Seed Health and Virology Laboratoryen_US
dc.typeToolen_US
dcterms.available2024-11-14en_US
dcterms.issued2024-11-14en_US

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