Molecular detection of Toxoplasma gondii and Sarcocystis spp. co-infection in Tunisian Merguez, a traditional processed sausage beef meat

cg.contactamairia.safa@gmail.comen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerManouba University, National School of Veterinary Medicine of Sidi Thabet - ENMVen_US
cg.contributor.centerInstitute of Veterinary Researches of Tunisia - IRVTen_US
cg.contributor.centerNational Institute for Agrarian and Veterinary Research - INIAV IPen_US
cg.contributor.centerUniversity of Lisbon, Faculty of Veterinary Medicine, Centre for Interdisciplinary Research in Animal Health - ULISBOA - FMV - CIISAen_US
cg.contributor.crpCGIAR Research Program on Livestock Agri-Food Systems - Livestocken_US
cg.contributor.funderCGIAR Fund Council - CGIAR - FCen_US
cg.contributor.projectCGIAR Research Program on Livestock Agri-Food Systemsen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryTNen_US
cg.coverage.geolocationLatitude: 40.16282955567599; Longitude: 21.650384277343772en_US
cg.coverage.regionNorthern Africaen_US
cg.creator.idRekik, Mourad: 0000-0001-7455-2017en_US
cg.date.embargo-end-dateTimelessen_US
cg.identifier.doihttps://dx.doi.org/10.1016/j.foodcont.2020.107618en_US
cg.isijournalISI Journalen_US
cg.issn0956-7135en_US
cg.journalFood Controlen_US
cg.subject.agrovoctunisiaen_US
cg.subject.agrovocpcren_US
cg.subject.agrovoctoxoplasma gondiien_US
cg.volume121en_US
dc.contributorRouatbi, Mariemen_US
dc.contributorRidha Frikha, Mohameden_US
dc.contributorGomes, Jacintoen_US
dc.contributorRekik, Mouraden_US
dc.contributorDarghouth, Mohamed Azizen_US
dc.contributorGharbi, Mohameden_US
dc.creatorAmairia, Safaen_US
dc.date.accessioned2021-01-12T23:51:32Z
dc.date.available2021-01-12T23:51:32Z
dc.description.abstractWith the increase of raw food consumption, there is a growing interest in the risk posed by zoonotic infections to human health. This study was performed to identify in popular processed beef meat-based sausages (Merguez) the prevalence of Toxoplasma gondii and the zoonotic species of Sarcocystis. A total of 99 samples, collected from four Tunisian regions, were used for molecular analyses. For each sample, DNA was extracted from 4 equal pieces and PCR reactions were performed. Phylogenetic analyses were performed to identify Sarcocystis species. Prevalences of T. gondii and Sarcocystis spp. DNA in Merguez samples were 47.5% (95% CI: 0.38–0.57) and 52.5% (95% CI: 0.43–0.62), respectively. Important differences between regions were found. The DNA of both parasites was detected in 14.1% of the same samples. For Sarcocystis spp. infection, the percentage of samples having one positive piece was significantly higher than those having two, three or four positive pieces. Whereas, for T. gondii, the majority of tested samples had two positive pieces (16.2%) (p = 0.002). Sequencing showed the presence of Sarcocystis tenella and Sarcocystis cruzi species. Our findings suggest a high prevalence of T. gondii and Sarcocystis spp. in Merguez sausages with a potential high human health risk if the latter are undercooked.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifier.citationSafa Amairia, Mariem Rouatbi, Mohamed Ridha Frikha, Jacinto Gomes, Mourad Rekik, Mohamed Aziz Darghouth, Mohamed Gharbi. (1/3/2021). Molecular detection of Toxoplasma gondii and Sarcocystis spp. co-infection in Tunisian Merguez, a traditional processed sausage beef meat. Food Control, 121.en_US
dc.identifier.statusTimeless limited accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/12318
dc.languageenen_US
dc.publisherElsevier (12 months)en_US
dc.sourceFood Control;121,(2020)en_US
dc.subjectsarcocystis sppen_US
dc.subjectsausageen_US
dc.subjectmerguezen_US
dc.titleMolecular detection of Toxoplasma gondii and Sarcocystis spp. co-infection in Tunisian Merguez, a traditional processed sausage beef meaten_US
dc.typeJournal Articleen_US
dcterms.available2020-09-10en_US
dcterms.issued2021-03-01en_US
mel.impact-factor5.548en_US
mel.project.openhttps://mel.cgiar.org/projects/237en_US

Files