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dc.contributorChaudhari, Sunilen_US
dc.creatorJanila, Pasupuletien_US
dc.date2019-07-01en_US
dc.date.accessioned2019-10-22T06:31:42Z
dc.date.available2019-10-22T06:31:42Z
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifier.citationPasupuleti Janila, Sunil Chaudhari. (1/7/2019). Genotyping of 10000 individuals annually to make early generation selection decisions.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/10346
dc.description.abstractGenotyping for FAD mutant alleles was initially done using allele-specific (AS-PCR) and cleaved amplified polymorphic sequence (CAPS) markers for detection of heterozygotes and homozygotes, respectively (Janila et al., 2016). This enabled better resource utilization but was cost intensive genotyping of one sample using CAPS marker costs 3.0 USD. The protocol for DNA isolation using seed chip is also standardized which will be helpful to minimize the resource required for genotyping (Figure 3). It reduces the time and resources required for planting and labeling individual plants in the field.en_US
dc.formatDOCXen_US
dc.languageenen_US
dc.rightsUnlicenseen_US
dc.subjectselection decision in early generationsen_US
dc.subjectcost-effective genotyping using snpsen_US
dc.titleGenotyping of 10000 individuals annually to make early generation selection decisionsen_US
dc.typeReporten_US
cg.creator.idJanila, Pasupuleti: 0000-0003-2583-9630en_US
cg.creator.idChaudhari, Sunil: 0000-0003-1441-3327en_US
cg.creator.ID-typeORCIDen_US
cg.creator.ID-typeORCIDen_US
cg.subject.agrovocgenotypingen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes and Dryland Cereals - GLDCen_US
cg.contributor.funderOPEC Fund for International Development - OFIDen_US
cg.contributor.projectEnhancing Groundnut Productivity and Profitability for Smallholder Farmers in Asia through Varietal Technologiesen_US
cg.contributor.project-lead-instituteInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.date.embargo-end-dateTimelessen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.regionSouth-Eastern Asiaen_US
cg.coverage.countryBDen_US
cg.coverage.countryINen_US
cg.coverage.countryLAen_US
cg.coverage.countryMMen_US
cg.coverage.countryVNen_US
cg.contactP.Janila@cgiar.orgen_US
dc.identifier.statusTimeless limited accessen_US
mel.project.openhttp://www.icrisat.org/en_US
mel.sub-typeDonor Reporten_US


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