Genotyping of 10000 individuals annually to make early generation selection decisions
cg.contact | P.Janila@cgiar.org | en_US |
cg.contributor.center | International Crops Research Institute for the Semi-Arid Tropics - ICRISAT | en_US |
cg.contributor.crp | CGIAR Research Program on Grain Legumes and Dryland Cereals - GLDC | en_US |
cg.contributor.funder | OPEC Fund for International Development - OFID | en_US |
cg.contributor.project | Enhancing Groundnut Productivity and Profitability for Smallholder Farmers in Asia through Varietal Technologies | en_US |
cg.contributor.project-lead-institute | International Crops Research Institute for the Semi-Arid Tropics - ICRISAT | en_US |
cg.coverage.country | BD | en_US |
cg.coverage.country | IN | en_US |
cg.coverage.country | LA | en_US |
cg.coverage.country | MM | en_US |
cg.coverage.country | VN | en_US |
cg.coverage.region | Southern Asia | en_US |
cg.coverage.region | South-Eastern Asia | en_US |
cg.creator.id | Janila, Pasupuleti: 0000-0003-2583-9630 | en_US |
cg.creator.id | Chaudhari, Sunil: 0000-0003-1441-3327 | en_US |
cg.date.embargo-end-date | Timeless | en_US |
cg.subject.agrovoc | genotyping | en_US |
dc.contributor | Chaudhari, Sunil | en_US |
dc.creator | Janila, Pasupuleti | en_US |
dc.date.accessioned | 2019-10-22T06:31:42Z | |
dc.date.available | 2019-10-22T06:31:42Z | |
dc.description.abstract | Genotyping for FAD mutant alleles was initially done using allele-specific (AS-PCR) and cleaved amplified polymorphic sequence (CAPS) markers for detection of heterozygotes and homozygotes, respectively (Janila et al., 2016). This enabled better resource utilization but was cost intensive genotyping of one sample using CAPS marker costs 3.0 USD. The protocol for DNA isolation using seed chip is also standardized which will be helpful to minimize the resource required for genotyping (Figure 3). It reduces the time and resources required for planting and labeling individual plants in the field. | en_US |
dc.format | DOCX | en_US |
dc.identifier | https://mel.cgiar.org/dspace/limited | en_US |
dc.identifier.citation | Pasupuleti Janila, Sunil Chaudhari. (1/7/2019). Genotyping of 10000 individuals annually to make early generation selection decisions. | en_US |
dc.identifier.status | Timeless limited access | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/10346 | |
dc.language | en | en_US |
dc.rights | Unlicense | en_US |
dc.subject | selection decision in early generations | en_US |
dc.subject | cost-effective genotyping using snps | en_US |
dc.title | Genotyping of 10000 individuals annually to make early generation selection decisions | en_US |
dc.type | Report | en_US |
dcterms.available | 2019-07-01 | en_US |
mel.project.open | http://www.icrisat.org/ | en_US |
mel.sub-type | Donor Report | en_US |